Characterization of the mIHF Gene of Mycobacterium smegmatis |
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Authors: | Marisa L Pedulla and Graham F Hatfull |
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Affiliation: | Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260 |
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Abstract: | Integration of mycobacteriophage L5 requires the mycobacterial integration host factor (mIHF) in vitro. mIHF is a 105-residue heat-stable polypeptide that is not obviously related to HU or any other small DNA-binding proteins. mIHF is most abundant just prior to entry into stationary phase and is essential for the viability of Mycobacterium smegmatis. |
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