Isolation of antimicrobial agent from the marine algae Cystoseira hakodatensis |
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| Authors: | Joon‐Young Jun Sosuke Nakajima Koji Yamazaki Yuji Kawai Hajime Yasui Yasuyuki Konishi |
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| Affiliation: | 1. Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan;2. Hokkaido Industrial Technology Center, 379, Hakodate, Hokkaido, Japan |
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| Abstract: | Cystoseira hakodatensis is an unutilised brown algae belonging to family Sargassaceae. A crude methanol extract from the algae showed inhibitory effects on the growths of Bacillus cereus and Bacillus licheniformis. To isolate the major antimicrobial agent, a sequential active‐guided isolation procedure was applied: liquid–liquid extraction, column chromatography and bio‐autography. A marked antimicrobial agent (active α) was isolated in hydrophobic fraction and was determined to phenolics without carbohydrates and proteins by phytochemical test. Regarding the antimicrobial potential, the isolated active α showed better inhibitory effects against B. cereus and B. licheniformis at 2 and 4 times of lower concentrations (62.5 and 31.3 μg mL?1) in comparison with epigallocatechin gallate. These results showed that C. hakodatensis is a potential source of antimicrobial agent capable of preventing the growth of the two bacteria. |
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| Keywords: | Algae antimicrobial agents
Cystoseira hakodatensis
isolation procedures pathogenic micro‐organisms polyphenols |
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