| 木瓜蛋白酶在[Cnmim]BF4-NaH2PO4双水相体系中的分配行为 |
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| 引用本文： | 蔡涛,张海德,董安华,彭健,吴蕊,朱英瑞.木瓜蛋白酶在[Cnmim]BF4-NaH2PO4双水相体系中的分配行为[J].化工学报,2015,66(6):2017-2022. |
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| 作者姓名： | 蔡涛 张海德 董安华 彭健 吴蕊 朱英瑞 |
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| 作者单位： | 海南大学食品学院, 海南 海口 570228 |
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| 基金项目： | 国家自然科学基金项目(31260401)。 |
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| 摘 要： | 为了研究木瓜蛋白酶在Cnmim]BF4-NaH2PO4双水相体系中的分配行为, 在298.15 K条件下利用浊点法测定了Cnmim]BF4-NaH2PO4双水相体系的双节线和液液相平衡数据, 建立了木瓜蛋白酶在该体系中的三维分配模型。利用Merchuk方程、Othmer-Tobias方程和Bancroft方程分别关联了双节线数据、液液相平衡数据, 拟合度分别在0.975、0.994以上, 结果满意。木瓜蛋白酶分配系数的对数与该体系上相的离子液体浓度和下相的盐浓度相关度都较高, 故通过Matlab建立了三者之间的分配模型, 实验值和预测值之间的相对偏差均在5%以内, 表明该模型能有效地预测木瓜蛋白酶在Cnmim]BF4-NaH2PO4双水相体系中的分配行为。
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| 关 键 词： | 木瓜蛋白酶 离子液体 萃取 双节线 液液相平衡 分配模型 蛋白质 |
| 收稿时间： | 2014-12-29 |
| 修稿时间： | 2015-03-06 |
Partition behavior of papain in [Cnmim]BF4-NaH2PO4 aqueous#br#
two-phase system |
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| CAI Tao,ZHANG Haide,DONG Anhua,PENG Jian,WU Rui,ZHU Yingrui.Partition behavior of papain in [Cnmim]BF4-NaH2PO4 aqueous#br#
two-phase system[J].Journal of Chemical Industry and Engineering(China),2015,66(6):2017-2022. |
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| Authors: | CAI Tao ZHANG Haide DONG Anhua PENG Jian WU Rui ZHU Yingrui |
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| Affiliation: | College of Food Science and Technology, Haikou 570228, Hainan, China |
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| Abstract: | To investigate the partition behavior of papain in the ionic liquids aqueous two-phase system containing Cnmim]BF4+NaH2PO4, the binodal curve and liquid-liquid equilibrium was measured by means of cloud point at T 298.15 K and a 3D theoretical model was established. The data fitting of binodal and the equilibrium was based on Merchuk, Othmer-Tobias and Bancroft equations with a high degree of fitting of above 0.975 and 0.994, respectively. Then, the correlation between logarithm of the partition coefficient and both the concentration of ionic liquids in upper phase and the salt concentration in lower phase was studied, and a partition model was established by Matlab. The mean relative deviations between the experimental and calculated data were <5%, which was sufficient to prove the reliability of this model. |
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| Keywords: | papain ionic liquids extraction binodal curve liquid-liquid equilibrium partition model protein |
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