| 中国南方稻田及其周边环境根结线虫种类鉴定 |
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| 引用本文： | 徐 幸,卓富彦,杨 芳,袁 斌,彭云良,郭 荣,姬红丽.中国南方稻田及其周边环境根结线虫种类鉴定[J].植物保护,2023,49(6):95-103. |
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| 作者姓名： | 徐 幸 卓富彦 杨 芳 袁 斌 彭云良 郭 荣 姬红丽 |
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| 作者单位： | 1. 农业农村部西南有害生物综合治理重点实验室, 四川省农业科学院植物保护研究所, 成都 610066; 2. 全国农业技术推广服务中心, 北京 100125; 3. 农业农村部华中作物有害生物综合治理重点实验室, 武汉 430064 |
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| 基金项目： | 四川省农业科学院“1+9”揭榜挂帅科技攻关项目（1+9KJGG006）；国家水稻产业技术体系（CARS-01-45）；四川省农业科学院科技成果中试熟化示范工程项目（CGZH2019GH03）；农业部华中作物有害生物综合治理重点实验室/农作物重大病虫草防控湖北省重点实验室开放基金课题（2020ZTSJJ4）；湖北省农业科技创新中心项目（2020-620-003-001） |
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| 摘 要： | 为了解根结线虫在中国南方稻区的分布和种类, 从15个省(直辖市/自治区)采集1 181份土样, 经生物测定发现312份土样中种植的水稻或番茄幼苗根系会产生根结, 阳性总检出率为26.42%。利用线虫特异性引物从171份阳性样品中鉴定出6种根结线虫:拟禾本科根结线虫Meloidogyne graminicola、北方根结线虫M.hapla、花生根结线虫M.arenaria、爪哇根结线虫M.javanica、象耳豆根结线虫M.enterolobii和南方根结线虫M.incognita, 分别占鉴定总样品数的88.89%、41.52%、29.82%、10.53%、4.09%和3.51%。被1、2、3、4、5、6种根结线虫混合侵染的土样比例分别为42.11%、41.52%、12.87%、2.92%、0.58%和0, 其中最常见的为拟禾本科根结线虫单独侵染(34.50%)、拟禾本科根结线虫与北方根结线虫(22.81%)或花生根结线虫(13.45%)混合侵染。本研究结果将为制定相应的传播阻断策略和防控措施提供参考。
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| 关 键 词： | 稻田 拟禾本科根结线虫 混合侵染 生物测定 特异性引物 |
| 收稿时间： | 2022/9/28 0:00:00 |
| 修稿时间： | 2022/12/15 0:00:00 |
Identification of root-knot nematode species from paddy fields and surrounding environment in southern China |
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| XU Xing,ZHUO Fuyan,YANG Fang,YUAN Bin,PENG Yunliang,GUO Rong,JI Hongli.Identification of root-knot nematode species from paddy fields and surrounding environment in southern China[J].Plant Protection,2023,49(6):95-103. |
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| Authors: | XU Xing ZHUO Fuyan YANG Fang YUAN Bin PENG Yunliang GUO Rong JI Hongli |
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| Affiliation: | 1. Key Laboratory of Integrated Management of Pests on Crops in Southwest China, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 2. National Agro-Tech Extension and Service Center, Beijing 100125, China; 3. Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture and Rural Affairs, Wuhan 430064, China |
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| Abstract: | To investigate the root-knot nematode (RKN) species in rice production areas in southern China, 1 181 soil samples were taken from 15 provinces (municipality/autonomous region). Totally 312 samples were confirmed to form root galls on the roots of rice or tomato. The positive ratio was 26.42%. Six species of RKNs were identified from 171 soil samples by PCR amplification using specific primers. In the identified samples, the detection ratio of Meloidogyne graminicola, M.hapla, M.arenaria, M.javanica, M.enterolobii, and M.incognita was 88.89%, 41.52%, 29.82%, 10.53%, 4.09% and 3.51%, respectively. The ratio of soil samples infested by one, two, three, four, five and six species of RKNs was 42.11%, 41.52%, 12.87%, 2.92%, 0.58% and 0, respectively. The most common infestation in all soil samples was by M.graminicola alone (34.50%), followed by mixed infestation with M.hapla(22.81%) and M.arenaria (13.45%). Our study provides valuable references for the prevention and control of rice root-knot nematodes. |
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| Keywords: | rice field Meloidogyne graminicola mixed infestation bioassay specific primers |
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