| 外周血液中miRNA表达与老年性黄斑变性相关性研究 |
| |
| 引用本文： | 朱美江,任成达,蔡雯婷,梁秀玮,金惠子,张瑞玲,刘畅,范佳淇,沈天怡,于靖.外周血液中miRNA表达与老年性黄斑变性相关性研究[J].眼科新进展,2019,0(7):677-681. |
| |
| 作者姓名： | 朱美江 任成达 蔡雯婷 梁秀玮 金惠子 张瑞玲 刘畅 范佳淇 沈天怡 于靖 |
| |
| 作者单位： | 200072 上海市，同济大学附属第十人民医院眼科 |
| |
| 摘 要： | 目的 检测微小核糖核酸（microribonucleicacids，miRNA）在老年性黄斑变性（age-related macular degeneration，AMD）患者中的表达，并探讨miRNA的表达量与AMD病程之间的关系。方法 选取2014年1月至2016年11月于同济大学附属第十人民医院眼科门诊就诊的AMD患者6例为试验组，并选取同期6名正常人为对照组，通过基因芯片技术检测两组血液中miRNA的表达量。扩大样本的病例对照研究中共纳入126例AMD患者和140名正常人，检测其血液样本中miRNA的表达，比较两组人群间miRNA的表达量差异。结果 通过基因芯片技术，在试验组与对照组间共检测出216个miRNA存在表达差异（均为P<0.05），与对照组相比，试验组中111个miRNA表达量上升，105个miRNA表达量下降，差异均有统计学意义（均为P<0.05）。扩大样本的病例对照研究结果表明，在AMD患者中，miR-27a-3p、miR-29b-3p、miR-195-5p的表达量显著上升，同时，湿性AMD患者血液中miR-27a-3p的表达量高于干性AMD患者，差异均有统计学意义（均为P<0.05）。结论 AMD患者外周血中miRNA表达量水平有明显变化，miR-27a-3p、miR-29b-3p、miR-195-5p可能成为AMD血清学诊断和预后的标志物。
|
| 关 键 词： | 老年性黄斑变性 miRNA 基因芯片技术 病例分析 |
Correlation between the expression of circulating miRNAs and age-related macular degeneration |
| |
| ZHU Mei-Jiang,REN Cheng-Da,CAI Wen-Ting,LIANG Xiu-Wei,JIN Hui-Zi,ZHANG Rui-Ling,LIU Chang,FAN Jia-Qi,SHEN Tian-Yi,YU Jing.Correlation between the expression of circulating miRNAs and age-related macular degeneration[J].Recent Advances in Ophthalmology,2019,0(7):677-681. |
| |
| Authors: | ZHU Mei-Jiang REN Cheng-Da CAI Wen-Ting LIANG Xiu-Wei JIN Hui-Zi ZHANG Rui-Ling LIU Chang FAN Jia-Qi SHEN Tian-Yi YU Jing |
| |
| Affiliation: | Department of Ophthalmology，Tenth People’s Hospital of Tongji University，Shanghai 200072，China |
| |
| Abstract: | Objective To detect the expression of microribonucleic acid (miRNA) inpatients with age-related macular degeneration (AMD),and to investigate the relationship between the expression of miRNA and the progression of AMD.Methods Totally six AMD patients as experimental group and six gender-matched control patients were included in our study in the outpatient department of the Tenth People’s Hospital of Tongji University between January 2014 and November 2016,miRNA expression was measured by Taqman between these two groups.Furthermore,the larger whole blood samples of 126 AMD patients and 140 control patients were obtained in a case-control study to detect the differences of miRNA expression in the two groups.Results A total of 216 differentially expressed miRNAs were detected via gene chips microarray in experimental group and control group (all P<0.05).Compared with control group,the expression of 111 miRNAs increased and 105 miRNAs decreased in experimental group.The results showed that the expression of miR-27a-3p,miR-29b-3p and miR-195-5p were significantly higher in AMD patients in the expanded case-control study.Moreover,the level of miR-27a-3p was higher in patients with wet AMD compared to patients with dry AMD (all P<0.05).Conclusion Circulating miRNA levels are significantly varied in AMD patients.Especially,miR-27a-3p,miR-29b-3p and miR-195-5p may be a marker of serological diagnosis and prognosis of AMD. |
| |
| Keywords: | age-related macular degeneration miRNA microarray case-control |
|
| 点击此处可从《眼科新进展》浏览原始摘要信息 |
|
点击此处可从《眼科新进展》下载全文 |
|
