| 后部巩膜F4/80和CD206阳性巨噬细胞在小鼠形觉剥夺性近视发生发展中的变化 |
| |
| 引用本文： | 李东辉,龙琴,王静怡,陈晨.后部巩膜F4/80和CD206阳性巨噬细胞在小鼠形觉剥夺性近视发生发展中的变化[J].眼科新进展,2019,0(12):1116-1119. |
| |
| 作者姓名： | 李东辉 龙琴 王静怡 陈晨 |
| |
| 作者单位： | 100730 北京市，中国医学科学院，北京协和医学院，北京协和医院眼科 |
| |
| 摘 要： | 目的 研究后部巩膜F4/80+和CD206+巨噬细胞在小鼠形觉剥夺性近视发生发展中的变化趋势。方法 将24只（48眼）4周龄C57BL/6小鼠按随机数字表法分别接受形觉剥夺处理和无处理（各12只），研究分为3组:形觉剥夺组（单眼遮盖，n=12），自身对照组（对侧眼无遮盖，n=12），正常对照组（无处理组右眼，n=12）。实验前（0 d）进行屈光度检测；于实验14 d和28 d测量屈光度后分别处死6只小鼠，分离眼球测量眼轴，并分离后部巩膜组织，采用流式细胞术检测后部巩膜组织中F4/80和CD206的表达，观察各指标的动态变化和内在关联。结果 实验0 d，形觉剥夺组、自身对照组和正常对照组的屈光度差异均无统计学意义（均为P>0.05）。实验14 d，3组屈光度和眼轴差异均无统计学意义（均为P>0.05）；形觉剥夺组后部巩膜F4/80+巨噬细胞以及F4/80+、F4/80+/CD206+巨噬细胞的百分比均显著高于自身对照组（均为P<0.05）。实验28 d，形觉剥夺组与自身对照组比较，屈光度明显向近视偏移（P=0.001）；并伴有眼轴的显著延长（P=0.02）；形觉剥夺组后部巩膜F4/80+巨噬细胞以及F4/80+/CD206+巨噬细胞的百分比均显著高于自身对照组（均为P<0.05）。实验28 d，形觉剥夺组小鼠后部巩膜F4/80+/CD206+巨噬细胞的百分比显著高于实验14 d时（P=0.02）。实验28 d，形觉剥夺组后部巩膜F4/80+/CD206+巨噬细胞的百分比和屈光度呈显著负相关（r=-0.89，P=0.02），和眼轴呈显著正相关（r=0.98，P=0.001）。各时间点自身对照组和正常对照组的各指标差异均无统计学意义（均为P>0.05）。结论 C57BL/6小鼠形觉剥夺性近视的发生和发展中存在后部巩膜组织F4/80+/CD206+巨噬细胞的上调，与屈光度和眼轴有相关性，提示F4/80+/CD206+巨噬细胞参与近视的发生发展过程。
|
| 关 键 词： | 形觉剥夺 近视 巨噬细胞 巩膜 |
Changes in F4/80 and CD206 positive macrophages in posterior sclera in the development of form-deprivation myopia of mice |
| |
| LI Dong-Hui,LONG Qin,WANG Jing-Yi,CHEN Chen.Changes in F4/80 and CD206 positive macrophages in posterior sclera in the development of form-deprivation myopia of mice[J].Recent Advances in Ophthalmology,2019,0(12):1116-1119. |
| |
| Authors: | LI Dong-Hui LONG Qin WANG Jing-Yi CHEN Chen |
| |
| Affiliation: | Department of Ophthalmology，Peking Union Medical College Hospital，Peking Union Medical College，Chinese Academy of Medical Sciences，Beijing 100730，China |
| |
| Abstract: | Objective To investigate the dynamic expression levels of F4/80+ and CD206+ macrophages in the posterior sclera in mice with form-deprivation myopia.Methods Twenty four C57BL/6 mice (48 eyes)，4-week old，undergoing monocular form-deprivation (12 mice) and untreatment (12 mice)，were divided into 3 groups according to random number table:form deprivation group (mice with monocular form-deprivation)；self-control group (fellow eyes without form deprivation) and normal control group (no treatment).The refractive diopter of mice was examined before operation，on day 14 and 28.Then 6 mice were sacrificed，and the axial length，the expression levels of F4/80+ and CD206+ in the posterior sclera were examined by flow cytometry on day 14 and 28，respectively.The dynamic changes and internal relations of above parameters were investigated.Results There were no significant differences among the 3 groups in terms of refractive diopter (all P>0.05).On day 14，there were no significant differences in refractive diopter and axial length in the three groups (all P>0.05).In the form deprivation group，F4/80+ macrophages and the percentage of F4/80+，F4/80+/CD206+ macrophages were significantly higher than those of the self-control group (all P<0.05).At 28 d，form deprivation group showed significantly myopic shift (P=0.001) accompanied with elongated axial length (P=0.02) compared with self-control group；in the form deprivation group，F4/80+ macrophages and the percentage of F4/80+，F4/80+/CD206+ macrophages were significantly higher than those of the self-control group (all P<0.05).In the form deprivation group，F4/80+/CD206+ macrophages in mouse posterior sclera at 14 d was significantly higher compared with that of 28 d (P=0.02).The expression of F4/80+/CD206+ was negatively correlated with refractive diopter (r=-0.89，P=0.02) and positively correlated with axial length (r=0.98，P=0.001) on day 28.There were no significant differences in the indexes between the self-control group and the normal control group at each time point (all P>0.05).Conclusion The expression of F4/80+/CD206+ macrophage is shown to be up-regulated and correlated with refractive diopter and axial length in mouse posterior sclera in form-deprivation myopia，which implies that F4/80+/CD206+ macrophage maybe involves in the development of myopia. |
| |
| Keywords: | form deprivation myopia macrophage sclera |
|
| 点击此处可从《眼科新进展》浏览原始摘要信息 |
|
点击此处可从《眼科新进展》下载全文 |
