Monitoring dynamic alterations in calcium homeostasis by T1‐mapping manganese‐enhanced MRI (MEMRI) in the early stage of small intestinal ischemia–reperfusion injury |
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| Authors: | Da‐wei Zhao Le‐tian Zhang Hai‐yun Cheng Yu‐long Zhang Jia‐yan Min Hua‐liang Xiao Yi Wang |
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| Affiliation: | 1. Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China;2. Department of Pathology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China |
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| Abstract: | Manganese‐enhanced MRI studies have proven to be useful in monitoring physiological activities associated with calcium ions (Ca2+) due to the paramagnetic property of the manganese ion (Mn2+), which makes it an excellent probe of Ca2+. In this study, we developed a method in which a Mn2+‐enhanced T1‐map MRI could enable the monitoring of Ca2+ influx during the early stages of intestinal ischemia–reperfusion (I/R) injury. The Mn2+ infusion protocol was optimized by obtaining dose‐dependent and time‐course wash‐out curves using a Mn2+‐enhanced T1‐map MRI of rabbit abdomens following an intravenous infusion of 50 mmol/l MnCl2 (5–10 nmol/g body weight (BW)). In the rabbit model of intestinal I/R injury, T1 values were derived from the T1 maps in the intestinal wall region and revealed a relationship between the dose of the infused MnCl2 and the intestinal wall relaxation time. Significant Mn2+ clearance was also observed over time in control animals after the infusion of Mn2+ at a dose of 10 nmol/g BW. This technique was also shown to be sensitive enough to monitor variations in calcium ion homeostasis in vivo after small intestinal I/R injury. The T1 values of the intestinal I/R group were significantly lower (P < 0.05) than that of the control group at 5, 10, and 15 min after Mn2+ infusion. Our data suggest that MnCl2 has the potential to be an MRI contrast agent that can be effectively used to monitor changes in intracellular Ca2+ homeostasis during the early stages of intestinal I/R injury. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | MRI manganese chloride small intestine ischemia– reperfusion injury calcium channel manganese‐enhanced MRI T1 mapping calcium homeostasis |
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