| 鼠李糖乳杆菌对产后抑郁小鼠行为和运动能力的影响 |
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| 引用本文： | 李腾,周小兰,褚静,王立芹,曹国芬,张海苗.鼠李糖乳杆菌对产后抑郁小鼠行为和运动能力的影响[J].蚌埠医学院学报,2021,46(3):295-299. |
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| 作者姓名： | 李腾 周小兰 褚静 王立芹 曹国芬 张海苗 |
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| 作者单位： | 西安医学院护理学院 妇产科护理学教研室, 陕西 西安 710021 |
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| 基金项目： | 陕西省教育厅专项科学研究项目 |
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| 摘 要： | 目的探讨鼠李糖乳杆菌对小鼠产后抑郁样行为和运动能力的影响。方法在小鼠妊娠期间施用地塞米松磷酸钠建立产后抑郁症（PPD）小鼠模型。将受孕的25只小鼠随机分为:鼠李糖乳杆菌干预低剂量组（Ⅰ组）、鼠李糖乳杆菌干预高剂量组（Ⅱ组）、阳性对照组（Ⅲ组）、模型对照组（Ⅳ组）和空白对照组（Ⅴ组）。通过灌胃给药的方式进行干预，Ⅰ组和Ⅱ组小鼠分别给予鼠李糖乳杆菌1×107 CFU/（kg·d）和1×108 CFU/（kg·d），Ⅲ组小鼠给予1.8 mg·kg-1·d-1帕罗西汀，Ⅳ组和Ⅴ组小鼠给予等量的0.9%氯化钠溶液，干预时间为4周。通过24 h食物消耗实验、黑白箱实验、强迫游泳实验和悬尾实验检测各组小鼠的行为学表现。结果造模前各组小鼠摄食量、体质量变化率、白箱停留时间和黑白箱穿梭次数、不动状态持续时间和悬尾不动时间方面，差异均无统计学意义（P>0.05）。与造模前相比，造模后和干预后各组小鼠摄食量减少，体质量变化率增加（P < 0.05）；造模后和干预后Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组小鼠强迫游泳不动持续时间和悬尾不动时间增加。与造模后相比，干预后各组小鼠体质量变化率增加（P < 0.05），干预后Ⅰ组、Ⅱ组和Ⅲ组小鼠强迫游泳不动持续时间和悬尾不动时间减少。造模后与Ⅴ组相比，其余组小鼠黑白箱穿梭次数有所减少，悬尾不动时间升高（P < 0.05）。干预后黑白箱穿梭次数各组间差异均无统计学意义（P>0.05）；干预后与Ⅴ组比较，其余组小鼠的悬尾不动时间增加（P < 0.01）；与Ⅳ组比较，Ⅰ组、Ⅱ组和Ⅲ组的悬尾不动时间减少（P < 0.01）。结论雌性小鼠妊娠期间施用地塞米松磷酸钠表现出产后抑郁样行为，鼠李糖乳杆菌干预后对产后小鼠的焦虑抑郁和运动能力有一定改善。
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| 关 键 词： | 抑郁症 鼠李糖乳杆菌 产后行为 运动能力 小鼠 |
| 收稿时间： | 2019-12-30 |
Effect of Lactobacillus rhamnosus on the behavior and motor ability of postpartum depression mice |
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| LI Teng,ZHOU Xiao-lan,CHU Jing,WANG Li-qin,CAO Guo-fen,ZHANG Hai-miao.Effect of Lactobacillus rhamnosus on the behavior and motor ability of postpartum depression mice[J].Journal of Bengbu Medical College,2021,46(3):295-299. |
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| Authors: | LI Teng ZHOU Xiao-lan CHU Jing WANG Li-qin CAO Guo-fen ZHANG Hai-miao |
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| Affiliation: | Department of Obstetrics and Gynecology Nursing, Xi'an Medical Univercity, Xi'an Shanxi 710021, China |
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| Abstract: | ObjectiveTo investigate the effecs of Lactobacillus rhamnosus on behavior and motor ability of postpartum depression-like mice.MethodsThe model of postpartum depression(PPD) was established using dexamethasone sodium phosphate during pregnancy in mice.Twenty-five pregnant mice were randomly divided into the low dose group(group Ⅰ), high dose group (group Ⅱ), positive control group(group Ⅲ), model control group(group Ⅳ) and blank control group(group Ⅴ).All mice were intervened by intragastric administration of drugs.The group Ⅰ and group Ⅱ were intervened using 1×107 and 1×108 CFU(kg·d) of Lactobacillus rhamnosus, respectively, the group Ⅲ were intervened using 1.8 mg·kg-1·d-1 of paroxetine, and the group Ⅳ and group Ⅴ mice were intervened using the same amount of 0.9% sodiun chloride solution for 4 weeks.The behaviors of mice in each group were tested using 24-hour food consumption experiment, black-and-white box experiment, forced swimming experiment and tail suspension experiment.ResultsThe differences of the intake, body mass change rate, white box stay time and black-white box shuttle times, duration of immobility and time of suspension tail immobility among five groups were not statistically significant before making model(P>0.05).Compared with before making model, the rats' intake decreased, and the rate of body mass change increased after making model and intervention(P < 0.05).After making model and intervention, the duration of forced swimming and time of suspension tail immobility increased in group Ⅰ, group Ⅱ, group Ⅲ and group Ⅳ.Compared with after making model, the body mass change rate of mice in the intervention group increased(P < 0.05), and the duration of forced swimming and tail suspension immobility decreased in the group Ⅰ, group Ⅱ and group Ⅲ after intervention.Compared with group Ⅴ after making model, the number of black-white box shuttle in other groups decreased, and the time of suspension tail immobility was significantly increased(P < 0.05).After intervention, the differences of the number of black-white box shuttle among five groups were not statistically significnat(P>0.05).Compared with group Ⅴ after intervention, the suspension tail immobile time in other groups increased(P < 0.01).Compared with group Ⅳ after intervention, the suspension tail immobile time in the group Ⅰ, group Ⅱ and group Ⅲ decreased(P < 0.01).ConclusionsThe female mice treated with dexamethasone sodium phosphate during pregnancy show postpartum depression-like behavior, and Lactobacillus rhamnosus intervention can improve the postpartum anxiety, depression and exercise ability of postnatal mice. |
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