| 抗TMV普通烟中N导入片段的特异分子标记开发与交换单株筛选 |
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| 引用本文： | 刘勇,黄昌军,陈姝敏,肖炳光,于海芹,袁诚,曾建敏,曹培健,李永平.抗TMV普通烟中N导入片段的特异分子标记开发与交换单株筛选[J].中国烟草学报,2022,28(3):89-95. |
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| 作者姓名： | 刘勇 黄昌军 陈姝敏 肖炳光 于海芹 袁诚 曾建敏 曹培健 李永平 |
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| 作者单位： | 1.云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心，昆明，650021 |
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| 基金项目： | 中国烟草总公司云南省公司科技项目“利用短N导入片段材料定向改良云烟87品种TMV抗性”2018530000241003 |
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| 摘 要： | 背景和目的 N导入片段赋予烟草品种对烟草花叶病毒（Tobacco mosaic virus，TMV）抗性，但同时带来产量低等连锁累赘，为了筛选减少连锁累赘的交换单株。 方法 以普通烟草中的N导入片段为材料，利用茄科作物基因组序列数据和比较基因组学方法，开发了特异扩增N导入片段的系列分子标记，通过分子标记在种质资源和分离群体中筛选交换单株。 结果 开发出23个N导入片段特异的分子标记，将携带N导入片段的普通烟种质划分为Coker176、Samsun NN和Xanthi nc三种长度类型。供试90份抗TMV的普通烟资源中，85份属于N导入片段长度相对较短的Coker176类型，未发现比Coker176更短的种质资源。利用N导入片段末端分子标记TN5.51，从不含N基因的K326、Y87、5F、HD与Coker176配置的4个回交分离群体的22572株抗TMV单株中，筛选到11株N导入片段交换单株，Coker176类型的N导入片段在普通烟中的交换频率为0.049%。其中24-8H等3个单株的N导入片段缩短约一半，具有减少连锁累赘的潜力。 结论 本研究获得了普通烟中N导入片段的系列分子标记和长度缩短一半的交换单株，为解析N导入片段连锁累赘的分子机理、减少连锁累赘奠定了基础。
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| 关 键 词： | 普通烟草 N导入片段 分子标记 交换单株 |
| 收稿时间： | 2021-12-15 |
The development of molecular markers of the N introgression segment in tobacco mosaic virus (TMV) resistant tobacco germplasms and exchange plant screening |
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| Affiliation: | 1.Yunnan Academy of Tobacco Agricultural Sciences, Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Kunming 650021, China2.Institute of Vegetables and Flowers, CAAS (Chinese Academy of Agricultural Sciences), Beijing 100081, China3.Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, Henan, China |
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| Abstract: | Many good disease-resistant genes in Solanaceae crop cultivars come from the chromosome introgression segment of wild species, but the non-target genome sequences on the exogenous chromosomes introgression segment may cause linkage drag, which limits the utilization of good genes of wild species. One of the well-known exogenous chromosome introgression segment in crop breeding is the N gene (N introgression segment) in Nicotiana tabacum introgressed from N. glutinosa. The N introgression segment endows resistance against tobacco mosaic virus (TMV) while causing linkage drag in tobacco leaf production. Objective To screen short N introgression segment exchange plant, Methods molecular markers specific to the N introgression segment were developed using the tomato genome as reference. Results A total of 23 N introgression segment specific molecular markers were developed. The markers are located in 3.50Mb ~ 5.77Mb of chromosome 11 of tomato reference genome. Using five N introgression segment specific markers and one N.tabacum specific markers, three length types of introgression segments were distinguished, named Coker176, Samsun NN and Xanthi nc. Eighty-five from 95 tested N.tabacum germplasms had relatively short introgression segments, which belong to the Coker176 type. No germplasm carrying shorter N segment than the Coker176 was found. Using the introgression segment end molecule mark TN5.51, 11 exchange plants were screened from 22572 plants from 4 separation groups inoculated with TMV to show necrotic lesion. The N introgression segment of the Coker176 length type has an average exchange frequency of 0.049% in N.tabacum. Among them, the N introgression segments of 24-8H and other 2 exchange plants were shortened by about half, showing the potential to reduce the linkage drags. Conclusion The series molecular markers specific to the N introgression segment and the exchange plants with half N introgression segment were developed, which provides a basis for analyzing the molecular mechanism of linkage drag and reducing the linkage drag. |
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