Biological responses in Balb/c mice after long‐term parenteral administration of the light subunit of mushroom tyrosinase |
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| Authors: | Wangsa T Ismaya Alida Efthyani Raymond R Tjandrawinata Heni Rachmawati |
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| Affiliation: | 1. Dexa Laboratories of Biomolecular Sciences, Cikarang, Indonesia;2. School of Pharmacy, Institut Teknologi Bandung, Bandung, Indonesia;3. Research Center for Nanosciences and Nanotechnology, Institut Teknologi Bandung, Bandung, Indonesia |
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| Abstract: | Light subunit of mushroom tyrosinase (LSMT) is a protein of unknown function from mushroom Agaricus bisporus that has been demonstrated to permeate through rat intestine ex vivo. Thus, it can be absorbed in the intestine, thereby holding a promise as a drug carrier for oral administration, similar to HA‐33 protein from botulinum, one of the closest structural homologs of LSMT. However, the safety of LSMT should be ensured prior to its use. Here, we described biological response of LSMT upon weekly intraperitoneal administration of 50 μg/day to the Balb/c mice for 12 weeks. Motoric and behavior profiles, as well as the index of main organs (liver, spleen, lung, heart, and kidney), and body weight, were not significantly changed as compared with the control group. Also, no IgG was detected in the serum. The results suggest that LSMT is safe for further development. |
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| Keywords: | immune‐tolerable protein light subunit of mushroom tyrosinase mushroom tyrosinase‐associated lectin‐like protein safety evaluation toxicology |
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