| 钌多吡啶配合物诱导胃癌SGC-7901细胞凋亡 |
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| 引用本文： | 黄蓝仪,陈瑶,吴鹏,梁延华,李敏,邢德刚.钌多吡啶配合物诱导胃癌SGC-7901细胞凋亡[J].中国病理生理杂志,2019(8):1521-1525. |
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| 作者姓名： | 黄蓝仪 陈瑶 吴鹏 梁延华 李敏 邢德刚 |
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| 作者单位： | 广东药科大学 |
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| 基金项目： | 国家自然科学基金资助项目(No.31070585);广州市科技计划项目(No.201904010402);广东药科大学省级大学生创新创业训练计划项目(No.2017cxcy42) |
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| 摘 要： | 目的:观察钌多吡啶配合物Ru1对胃癌SGC-7901细胞凋亡的影响及其作用机制。方法:MTT检测细胞活性,并计算细胞活性抑制率;用结晶紫染色方法检测Ru1对SGC-7901细胞数量的抑制作用;通过AnnexinⅤ-FITC及PI染色法检测Ru1对SGC-7901细胞凋亡的影响;采用Western blot技术检测凋亡相关蛋白表达的情况。结果:MTT结果及结晶紫染色结果表明,Ru1能明显降低SGC-7901细胞活性及细胞数量;凋亡实验结果显示,Ru1作用于SGC-7901细胞24 h后,可导致SGC-7901细胞出现明显凋亡;Western blot结果显示Ru1可上调SGC-7901细胞Bax蛋白的表达,同时下调Bcl-2蛋白的表达(P<0.05)。结论:Ru1可以通过影响Bax蛋白和Bcl-2蛋白的表达从而诱导胃癌SGC-7901细胞凋亡。
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| 关 键 词： | 钌多吡啶配合物 SGC-7901细胞 细胞凋亡 胃癌 |
Effect of ruthenium-polypyridine complex on apoptosis of gastric cancer SGC-7901 cells |
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| HUANG Lan-yi,CHEN Yao,WU Peng,LIANG Yan-hua,LI Min,XING De-gang.Effect of ruthenium-polypyridine complex on apoptosis of gastric cancer SGC-7901 cells[J].Chinese Journal of Pathophysiology,2019(8):1521-1525. |
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| Authors: | HUANG Lan-yi CHEN Yao WU Peng LIANG Yan-hua LI Min XING De-gang |
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| Affiliation: | (Guangdong Pharmaceutical University, Guangzhou 510006, China) |
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| Abstract: | AIM: To study the effect of ruthenium-pyridine complex Ru1 on apoptosis of gastric cancer SGC-7901 cells. METHODS: MTT assay and crystal violet staining method were used to detect the viability and cell number of SGC-7901 cells treatment with Ru1. Annexin V-FITC and PI staining was performed to test the apoptosis rate of SGC-7901 cells. The protein expression of Bax and Bcl-2 was detected by Western blot. RESULTS: The results of MTT assay and crystal violet staining showed that the ruthenium-pyridine complexes significantly reduced the viability and cell number of SGC-7901 cells. Treatment with Ru1 for 24 h significantly increased the apoptotic rate of SGC-7901 cells(P<0.05). Ru1 up-regulated the expression of Bax protein and down-regulated the expression of Bcl-2 protein in the SGC-7901 cells(P<0.05). CONCLUSION: Ru1 induces apoptosis of SGC-7901 cells by affecting the expression of Bax and Bcl-2 proteins. |
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| Keywords: | Rruthenium-pyridine complexe SGC-7901 cells Apoptosis Gastric cancer |
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