| 人骨形态发生蛋白-2基因克隆及真核表达载体的构建 |
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| 引用本文： | 周诺,黄旋平,廖妮,韦山良,梁飞新,麦华明.人骨形态发生蛋白-2基因克隆及真核表达载体的构建[J].华西口腔医学杂志,2007,25(5):487-489. |
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| 作者姓名： | 周诺 黄旋平 廖妮 韦山良 梁飞新 麦华明 |
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| 作者单位： | 广西医科大学附属口腔医院口腔颌面外科, 广西南宁530021 |
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| 基金项目： | 国家自然科学基金
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广西自然科学基金 |
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| 摘 要： | 目的克隆人骨形态发生蛋白- 2（hBMP2）基因片段，构建pcDNA3.1- hBMP2真核表达质粒。方法采用逆转录聚合酶链式反应（RT- PCR）技术，从人骨肉瘤中扩增出人骨形态发生蛋白- 2基因片段，通过DNA重组技术将该基因片段重组于pcDNA3.1真核表达载体上，构建pcDNA3.1- hBMP2重组质粒，通过用PCR扩增、酶切电泳分析及DNA测序的方法对重组DNA进行鉴定。结果经PCR扩增、酶切电泳分析和DNA测序证实，本实验构建的重组质粒目的基因片段为人BMP2- cDNA。结论本实验成功克隆了hBMP2基因并构建成其真核表达质粒。
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| 关 键 词： | 骨形态发生蛋白- 2 基因克隆 真核表达载体 |
| 文章编号： | 1000-1182（2007）05-0487-03 |
| 收稿时间： | 2007-10-25 |
| 修稿时间： | 2007-01-23 |
Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector |
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| ZHOU Nuo,HUANG Xuan-ping,LIAO Ni,WEI Shan-liang,LIANG Fei-xin,MAI Hua-ming.Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector[J].West China Journal of Stomatology,2007,25(5):487-489. |
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| Authors: | ZHOU Nuo HUANG Xuan-ping LIAO Ni WEI Shan-liang LIANG Fei-xin MAI Hua-ming |
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| Affiliation: | Dept. of Oral and Maxillofacial Surgery, Hospital of Stomatology, Guangxi Medical University, Nanning 530021, China |
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| Abstract: | Objective To clone human bone morphogenetic protein-2(hBMP2) gene and construct its eukaryotic expression vector pcDNA3.1-hBMP2. Methods Human BMP2 gene was amplified by RT-PCR method from human osteosarcoma cells and constructed into eukaryotic expression vector pcDNA3.1-hBMP2. The gene in the vector pcDNA3.1-hBMP2 was identified by PCR amplification, enzyme digestion and DNA sequencing. Results The cloned DNA was confirmed to be hBMP-2 gene. Conclusion In this study, hBMP2 gene is successfully cloned and its eukaryotic expression vector pcDNA3.1-hBMP2 is constructed, which provides the foundation of using BMP2 gene therapy to accelerate new bone formation in distraction osteogenesis. |
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| Keywords: | bone morphogenetic protein-2 gene clone eukaryotic expression vector |
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