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| 中国部分食品分离单增李斯特菌的抗菌药物敏感性及耐药基因检测 | |
| 引用本文： | 王天姝,王艳,贺春月,叶正兴,王毅,许柯,俞骅,汪永禄,张兰荣.中国部分食品分离单增李斯特菌的抗菌药物敏感性及耐药基因检测[J].疾病监测,2013,28(3):224-229. |
| 作者姓名： | 王天姝 王艳 贺春月 叶正兴 王毅 许柯 俞骅 汪永禄 张兰荣 |
| 作者单位： | 1. 贵阳医学院微生物学教研室,贵州贵阳550004;中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室 2. 中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室 3. 贵阳医学院微生物学教研室,贵州贵阳,550004 4. 浙江省杭州市疾病预防控制中心 5. 安徽省马鞍山市疾病预防控制中心 6. 北京市通州区疾病预防控制中心 |
| 基金项目： | 国家重大传染病防治专项课题 |
| 摘 要： | 目的 了解我国部分食品中分离单增李斯特菌的抗菌药物敏感性和耐药性,探讨单增李斯特菌耐药基因的携带与耐药表型的关系。 方法 采用微量肉汤稀释法对2005-2011年从我国7个省市/地区食品分离的203株单增李斯特菌进行庆大霉素、氨苄西林、头孢噻吩、青霉素、四环素、强力霉素、亚胺培南、红霉素、环丙沙星、左氧氟沙星、利福平、万古霉素、氯霉素、氨苄西林/舒巴坦、复方新诺明、诺氟沙星、头孢呋肟、多粘菌素B、呋喃妥因、氯林可霉素共20种抗菌药物药敏试验,采用PCR方法对实验菌株进行9种耐药基因及接合型转座子Tn916检测,并对阳性基因产物进行序列测定分析。 结果 食源性单增李斯特菌对多粘菌素B、呋喃妥因、头孢呋肟、氯林可霉素、四环素、强力霉素、环丙沙星、诺氟沙星和氯霉素的耐药率分别为98.52%、55.66%、50.25%、12.81%、2.46%、1.97%、0.99%、0.99%和0.49%。203株单增李斯特菌中,5株对四环素耐药的菌株均检测出tetM基因,其中3株菌的tetM基因位于接合型转座子Tn916上。 结论 食品中分离的单增李斯特菌存在不同程度的耐药情况,并有83株多重耐药株存在,具有引起食源性单增李斯特菌感染的风险存在,应加强对抗生素使用的管理以保证食品安全及公众健康。 |
| 关 键 词： | 单增李斯特菌 微量肉汤稀释法 接合型转座子Tn916 |
| 收稿时间： | 2012-11-28 |
Detection of drug susceptibility and resistant genes in selected food borne Listeria monocytogens in China |
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| Abstract: | Objective To understand the drug susceptibility and relationship between antibiotic resistant genes and resistant phenotype in selected food borne Listeria monocytogenes strains in China. Methods The drug susceptibility of 203 food borne Listeria monocytogenes strains was detected by broth micro dilution test as recommended by Clinical and Laboratory Standards Institute. A total of 20 antibiotics, including gentamicin, ampicillin, penicillin, tetracycline, doxycycline, imipenem, erythromycin, ciprofloxacin, levofloxacin, cephalothin, rifampin, vancomycin, chloramphenicol, trimethoprim-sufamethoxazole, ampicillin-sulbactam, norfloxacin, polymyxin B sulfate, nitrofurantoin, clindamycin and cefuroxime, were used in the drug susceptibility test. Polymerase chain reaction(PCR) was conducted to detect 9 resistance genes and transposon Tn916. Results The resistant rates of the food borne Listeria monocytogenes strains to polymyxin B sulfate, nitrofurantoin, cefuroxime, clindamycin, tetracycline, doxycycline, ciprofloxacin, norfloxacin, chloramphenicol were 98.52%,55.66%,50.25%,12.81%,2.46%,1.97%,0.99%,0.99% and 0.49%, respectively. In the 203 food borne Listeria monocytogenes strains, tetM gene was only found in 5 tetracycline-resistant strains,3 of which were positive for Tn916. Conclusion Different levels of antibiotic resistance were detected in the tested food borne Listeria monocytogens, in which 83 strains were multi-drug resistant, demonstrating the risk of causing food borne Listeria monocytogenes infection Therefore, we should enhance the management of antibiotic use, insure food safety and improve public health. |
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