| 外源Ca~(2 )预处理对高温胁迫下辣椒叶片细胞膜透性和GSH、AsA含量及Ca~(2 )分布的影响 |
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| 引用本文： | 张宗申,利容千,王建波.外源Ca~(2 )预处理对高温胁迫下辣椒叶片细胞膜透性和GSH、AsA含量及Ca~(2 )分布的影响[J].植物生态学报,2001,25(2):230-234. |
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| 作者姓名： | 张宗申 利容千 王建波 |
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| 作者单位： | 武汉大学生命科学学院， 武汉 430072 |
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| 基金项目： | 湖北省自然科学基金 !(99J0 84) |
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| 摘 要： | 以辣椒 (Capsicum annuum)幼苗的叶片为材料 ,研究了外源 Ca2 预处理对热胁迫下细胞质膜透性和谷胱甘肽 (GSH)、抗坏血酸 (As A)含量变化及 Ca2 分布的影响。结果表明 :外源 Ca2 预处理能减轻热胁迫引起的细胞膜破坏 ,能够减少叶片中 GSH和 As A的破坏。热胁迫后 ,Ca2 具有从胞外转运到胞质内和叶绿体中的趋势 ;外施Ca2 预处理能够明显增加细胞间隙、液泡和叶绿体中的 Ca2 颗粒密度 ,能够稳定热胁迫下叶肉细胞膜和叶绿体的超微结构。结果表明 ,外施 Ca2 预处理可能通过改变细胞内外的 Ca2 分布 ,减轻热胁迫对叶肉细胞的伤害
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| 关 键 词： | 辣椒 热胁迫 细胞膜透性 Ca~(2 ) |
EFFECTS OFCa~(2 ) PRETREATMENT ON PLASMALEMMA PERMEABILITY, GSH AND ASA CONTENTS, AND CALCIUM DISTRIBUTION IN PEPPER MESOPHYLL CELLS UNDER HEAT STRESS |
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| Abstract: | In this study, young pepper (Capsi cum annuum L. cv. Xiang-Yan10)leaves were used to examine the effects of exo genous Ca2+ pretreatment on electrolyte leakage, ascorbate(AsA) and glutat hione(GSH) contents, and the change in Ca2+ distribution in mesophyll cell s under heat stress. The results show that exogenous Ca2+ pretreatment can alleviate the damage of cell membrane caused by heat stress. The amount of GSH and AsA in calcium-treated leaves under heat stress was higher than that in con trol leaves. In the control pepper seedlings growing under normal temperature, deposits of calcium antimonate, an indicator for Ca2+ localization, were ob served within the vacuoles, intercellular spaces, and chloroplasts. This indicat es that Ca2+ pretreatment could induce changes in Ca2+ distribution and significantly increase the Ca2+ level in intercellular spaces, vacuole s and chloroplasts. When calcium-treated leaves were under heat stress at 40°, the amount of Ca2+ located in intercellular space decreased, and most of t hem transferred to cytosol and chloroplast across the plasmalemma. The results s uggest that exogenous Ca2+ results in changes in Ca2+ distribution b etween inter-and intracellular spaces and, as a result, the ultrastructure of m esophyll cells could be protected from damage caused by heat stress. |
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| Keywords: | Pepper Heat stress Plasmalemma permeability Ca~(2 ) |
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