| 脂多糖联合MPTP诱导的小鼠慢性帕金森病模型的评价 |
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| 引用本文： | 史肖锦,屈洪党.脂多糖联合MPTP诱导的小鼠慢性帕金森病模型的评价[J].实验动物与比较医学,2015,23(5):513-516. |
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| 作者姓名： | 史肖锦 屈洪党 |
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| 作者单位： | 蚌埠医学院第一附属医院神经内科, 安徽 蚌埠 233000;蚌埠医学院第一附属医院神经内科, 安徽 蚌埠 233000 |
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| 基金项目： | 蚌埠医学院研究生创新计划项目(Byycx1406);教育厅重点项目(KJ2014A163)。 |
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| 摘 要： | 目的 建立脂多糖(lipopolysaccharide, LPS)联合1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, MPTP)诱导的慢性小鼠帕金森病模型,探讨其行为学和黒质多巴胺能神经元细胞的变化情况。方法 将20只C57BL/6小鼠随机分为模型组和对照组,模型组每日腹腔注射LPS(0.25 mg/kg) 一次,连续3 d,最后一次注射LPS 4 h后,每日腹腔注射MPTP(25 mg/kg)一次,连续2 d,对照组腹腔注射相同量的生理盐水。8周后用步态分析和转棒实验评价小鼠的行为学能力,免疫组化法观察黑质酪氨酸羟化酶(TH) 阳性细胞变化情况。结果 模型组小鼠行为学变化较对照组差异有显著性(P<0.05),模型组小鼠中脑黒质区显示出严重的神经元细胞损伤。结论 LPS联合MPTP腹腔注射可成功诱导出慢性小鼠帕金森病模型,提示该模型可用于帕金森病的发病机制及药物治疗效果等相关研究。
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| 关 键 词： | LPS MPTP 帕金森病 小鼠模型 酪氨酸羟化酶 |
| 收稿时间： | 2015/5/20 0:00:00 |
Evaluation of a mouse model of Parkinson's disease induced by lipopolysaccharide plus MPTP |
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| SHI Xiao-jin and QU Hong-dang.Evaluation of a mouse model of Parkinson's disease induced by lipopolysaccharide plus MPTP[J].Laboratory Animal and Comparative Medicine,2015,23(5):513-516. |
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| Authors: | SHI Xiao-jin and QU Hong-dang |
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| Affiliation: | the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China;the First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China |
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| Abstract: | Objective The aim of this study was to establish a mouse model of chronic Parkinson''s disease induced by systemic administration of lipopolysaccharide plus 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP), and to study the changes of behavioral manifestation, numbers of dopaminergic neurons in the substantia nigra pars compacta. Methods Twenty C57BL mice were randomly divided into 2 groups: the saline control group and model group. The mice in the model group received three intraperitoneal (i.p.) injections of LPS (0.25 mg/kg), once daily for three consecutive days. Four hours following the final LPS injection, the mice received one subcutaneous injection of low-dose MPTP (25 mg/kg). The mice of control group were injected with the same volume of saline. Eight weeks later, the motor ability of the mice was evaluated by footprint test and rotarod test. The tyrosine hydroxylase (TH)-positive cells were observed by immunohistochemical analysis. Results Compared with the control group, the scores of behavioral test were significantly lower, numbers of TH immunoreactive cells were significantly less in the Parkinson''s model group (P<0.05). Conclusions Behavioral manifestation,number of dopaminergic neurons in the substantia nigra are significantly changed in the mouse models of Parkinson''s disease produced by repeated injection of LPS plus MPTP, suggesting that this chronic animal model can be used in the experimental study for pathogenesis and therapy of Parkinson''s disease. |
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| Keywords: | Lipopolysaccharide LPS MPTP Parkinson''s disease(PD) Rat model Tyrosine hydroxylase |
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