| 超高效液相色谱-串联质谱法测定猪肉中93种兽药和违禁化合物 |
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| 引用本文： | 姜文华,韦立毅,邱晋,尹伟丽,杨懿,孙成均.超高效液相色谱-串联质谱法测定猪肉中93种兽药和违禁化合物[J].现代预防医学,2020,0(8):1477-1483. |
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| 作者姓名： | 姜文华 韦立毅 邱晋 尹伟丽 杨懿 孙成均 |
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| 作者单位： | 1.四川大学华西公共卫生学院/华西第四医院，四川 成都 610041；2.四川仲测质量技术检测有限公司，四川 成都 610015；3. 西南医科大学公共卫生学院，四川 泸州 646000 |
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| 摘 要： | 目的 建立猪肉中93种兽药和违禁化合物残留的超高效液相色谱-串联质谱测定方法。方法 样品用0.005 mol/L EDTA-乙腈-甲醇（3∶1, v/v，含1%乙酸）混合溶液提取后，冷冻除酯，再经壳聚糖净化，经稀释后，用超高效液相色谱-串联质谱法测定。结果 方法线性范围为0.05～200.00 μg/L，定量限为0.266～50.100 μg/kg，回收率为64.6% ～118.6%，日内和日间RSD分别为0.50%～15.90%和0.18%～19.20%。结论 本法简便快速、灵敏、准确，可用于猪肉中93种兽药和违禁成分的测定。
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| 关 键 词： | 超高效液相色谱-串联质谱法 兽药残留 违禁成分 猪肉 |
Determination of 93 veterinary drugs and prohibited chemicals in pork by ultra high performance liquid chromatography-tandem mass spectrometry |
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| JIANG Wen-hua,WEI Li-yi,QIU Jin,YIN Wei-li,YANG Yi,SUN Cheng-jun.Determination of 93 veterinary drugs and prohibited chemicals in pork by ultra high performance liquid chromatography-tandem mass spectrometry[J].Modern Preventive Medicine,2020,0(8):1477-1483. |
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| Authors: | JIANG Wen-hua WEI Li-yi QIU Jin YIN Wei-li YANG Yi SUN Cheng-jun |
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| Affiliation: | *West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, Sichuan 610041, China |
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| Abstract: | Abstract: Objective To establish a method for the determination of 93 veterinary drugs and prohibited chemicals in pork by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Methods After the samples were extracted with 0.005 mol/L EDTA and acetonitrile-methanol (3:1, v/v, containing 1% acetic acid), then frozen for removing theft and then purified with chitosan. The purified solution was diluted, and the target analytes were determined by UHPLC-MS/MS. Results The linear ranges of the method were 0.05-200 μg/L. The limit of quantification was 0.266-50.1 μg/kg, and the recoveries were 64.6%-118.6%. The RSDs of intra-day and inter-day were 0.50%-15.9% and 0.18%-19.2%, respectively. Conclusion The method is simple, quick, sensitive and accurate. It can be used for the determination of 93 veterinary drugs and prohibited chemicals in pork. |
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| Keywords: | Ultra high performance liquid chromatography-tandem mass spectrometry Veterinary drug residues Prohibitedchemicals Pork |
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