| 微波辐射ELISA诊断日本血吸虫病的初步研究 |
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| 引用本文： | 顾启章,张宏莹,朱善济,徐文娟 指导者: 金国梁.微波辐射ELISA诊断日本血吸虫病的初步研究[J].中国寄生虫学与寄生虫病杂志,1999,17(2):115-116. |
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| 作者姓名： | 顾启章 张宏莹 朱善济 徐文娟 指导者: 金国梁 |
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| 作者单位： | 杭州医学高等专科学校，浙江省人民医院病理科 |
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| 摘 要： | 目的：探索高效快速的血吸虫病诊断方法。方法：用微波辐射ＥＬＩＳＡ和快速ＥＬＩＳＡ同步检测各期日本血吸虫病患者血清１１８份，健康人血清６１份，并殖吸虫病犬血清１２份。结果：两种方法的血吸虫病患者血清抗体阳性检出率分别为８８．１％（１０４／１１８）和９１．５％（１０８／１１８），无显著性差异。健康人血清抗体假阳性分别为２份（３．３％）和１份（１．６％），亦无显著性差异；交叉反应均无假阳性。结论：微波辐射ＥＬＩＳＡ具有与快速ＥＬＩＳＡ类似的敏感性和特异性，但前者具有快速获得检测结果的优点。
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| 关 键 词： | 血吸虫病 血清学诊断 抗体检测 微波辐射ELISA |
PRELIMINARY STUDIES ON MICROWAVE IRRADIATION ELISA FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA |
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| GU Qizhang,ZHANG Hongying,ZHU Shanji,XU WenjuanDirector: JIN Guoliang.PRELIMINARY STUDIES ON MICROWAVE IRRADIATION ELISA FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA[J].Chinese Journal of Parasitology and Parasitic Diseases,1999,17(2):115-116. |
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| Authors: | GU Qizhang ZHANG Hongying ZHU Shanji XU WenjuanDirector: JIN Guoliang |
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| Affiliation: | Hangzhou Medical College, Hangzhou 310012. |
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| Abstract: | AIM: To explore a fast and highly efficient method for the diagnosis of schistosomiasis japonica. METHODS: Using microwave irradiation ELISA (MWI-ELISA) and fast-ELISA to detect specific antibodies in sera from 118 cases with schistosomiasis japonica, 61 healthy individuals and 12 paragonimiasis cases. RESULTS: The positive rates of schistosomiasis cases were 88.1% (104/118) by MWI-ELISA and 91.5% (108/118) by fast-ELISA, respectively (chi 2 = 0.74, P > 0.05). The false positive reaction of healthy individuals was found in 2 cases(3.3%) by MWI-ELISA and 1 case (1.6%) by fast-ELISA, respectively (chi 2 = 0.34, P > 0.05). No false positive reaction was found in paragonimiasis cases. CONCLUSION: The sensitivity and specificity of the two tests were similar, however, MWI-ELISA was faster than fast-ELISA. |
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| Keywords: | Schistosomiasis serological diagnosis antibody detection microwave irradiation ELISA |
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