| miR-149-3p通过靶向S100A4抑制膀胱癌细胞的增殖、迁移和侵袭 |
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| 引用本文： | 谢顺明,王文晓,赵文武.miR-149-3p通过靶向S100A4抑制膀胱癌细胞的增殖、迁移和侵袭[J].现代肿瘤医学,2019,0(1):12-17. |
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| 作者姓名： | 谢顺明 王文晓 赵文武 |
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| 作者单位： | 德阳市人民医院泌尿外科，四川 德阳 618000 |
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| 基金项目： | 四川省科技计划项目(编号:2014SZ0023-2) |
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| 摘 要： | 目的:探讨miR-149-3p通过靶向S100A4抑制膀胱癌细胞的增殖、迁移和侵袭行为及其作用机制。方法:qPCR检测miR-149-3p的过表达情况；CCK-8细胞增殖实验检测miR-149-3p对膀胱癌细胞的增殖能力的影响；划痕愈合实验和Transwell侵袭实验检测miR-149-3p对膀胱癌细胞迁移和侵袭能力的影响；双荧光素酶报告基因检测miR-149-3p与S100A4的相互作用；Transwell侵袭实验检测S100A4的异位表达逆转miR-149-3p的抗肿瘤作用。结果:miR-149-3p过表达显著抑制培养的膀胱癌细胞48小时的生长能力；过表达miR-149-3p后在膀胱癌细胞中具有抗迁移和侵袭作用；miR-149-3p能与S100A4的3' UTR特异性结合，调控S100A4的表达活性；S100A4的过表达可以逆转由miR-149-3p引起的细胞迁移和侵袭能力的抑制。结论:miR-149-3p在膀胱癌的发生发展过程中起重要作用，可以通过靶向调节S100A4的表达影响膀胱癌细胞的增殖和迁移。
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| 关 键 词： | 膀胱癌 miR-149-3p S100A4 侵袭行为 |
miR-149-3p inhibits the proliferation，migration and invasion of bladder cancer cells by targeting S100A4 |
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| Xie Shunming,Wang Wenxiao,Zhao Wenwu.miR-149-3p inhibits the proliferation，migration and invasion of bladder cancer cells by targeting S100A4[J].Journal of Modern Oncology,2019,0(1):12-17. |
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| Authors: | Xie Shunming Wang Wenxiao Zhao Wenwu |
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| Affiliation: | Department of Urology Surgery，Deyang People's Hospital，Sichuan Deyang 618000，China. |
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| Abstract: | Objective:To investigate the inhibitory effect of miR-149-3p on the proliferation，migration and invasion of bladder cancer by targeting S100A4 and its mechanism.Methods:qPCR was used to detect the overexpression of miR-149-3p.The proliferation of bladder cancer cells was detected by CCK-8 cell proliferation assay.Scratch healing assay and Transwell invasion assay were used to detect the effect of miR-149-3p on the migration and invasion ability of bladder cancer cells.Dual luciferase reporter gene was used to detect miR-149-3p and S100A4 interaction.Transwell invasion assay examined the ectopic expression of S100A4 reversing the antitumor effect of miR-149-3p.Results:Overexpression of miR-149-3p significantly inhibited the growth of cultured bladder cancer cells for 48 hours.The miR-149-3p overexpression showed anti-migration and invasion in bladder cancer cells.miR-149-3p can specifically bind to the 3' UTR of S100A4 and regulate the expression of S100A4.Overexpression of S100A4 can reverse the inhibition of cell migration and invasion capacity caused by miR-149-3p.Conclusion:miR-149-3p plays an important role in the development and progression of bladder cancer.It may affect the proliferation and migration of bladder cancer cells by regulating the expression of S100A4. |
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| Keywords: | bladder cancer miR-149-3p S100A4 invasion behavior |
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