| Galectin-3调控Wnt信号通路对脑胶质瘤细胞凋亡的影响 |
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| 引用本文： | 尤 宇,李小旭,王重韧,赵 明,徐 欣,韩 磊,赵四军.Galectin-3调控Wnt信号通路对脑胶质瘤细胞凋亡的影响[J].现代肿瘤医学,2019,0(6):934-938. |
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| 作者姓名： | 尤 宇 李小旭 王重韧 赵 明 徐 欣 韩 磊 赵四军 |
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| 作者单位： | 郑州大学附属肿瘤医院（河南省肿瘤医院）神经外科，河南 郑州 450008 |
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| 基金项目： | 河南省医学科技攻关计划项目(编号:201503189) |
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| 摘 要： | 目的:半乳糖凝集素-3（Galectin-3）调控Wnt信号通路对人脑胶质瘤细胞凋亡的影响。方法:RT-PCR及Western blot检测人脑胶质瘤组织中Galectin-3的mRNA和蛋白表达；Western blot检测人脑胶质瘤U251、U87、SHG-44细胞中Galectin-3的蛋白表达；将Galectin-3的特异性siRNA(Galectin-siRNA)转染人脑胶质瘤U87细胞，Western blot、流式细胞术分别检测转染48 h后Galectin-3、Wnt5a、β-catenin和Cleaved caspase3蛋白表达及细胞凋亡率。结果:Galectin-3在人脑胶质瘤组织mRNA和蛋白表达均显著高于瘤旁组织（P＜0.01）；U251、U87、SHG-44细胞中Galectin-3蛋白表达从高到低为U87>U251>SHG-44，选择U87细胞作为后续研究；Galectin-3-siRNA1的Galectin-3蛋白表达最低，选择作为后续研究；NC-siRNA组细胞凋亡率、Cleaved caspase3、Wnt5a、β-catenin蛋白表达与对照组差异不显著（P＞0.05），与对照组比较，Galectin-3-siRNA组细胞凋亡率明显升高，Cleaved caspase3蛋白表达明显升高，Wnt5a和β-catenin蛋白表达明显降低（P＜0.01）。结论:沉默Galectin-3表达可诱导人脑胶质瘤细胞凋亡，机制可能与Wnt信号通路的下调有关。
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| 关 键 词： | Galectin-3 Wnt信号通路 脑胶质瘤 凋亡 |
The effect of Galectin-3 regulated Wnt signaling pathway on apoptosis of human glioma cells |
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| You Yu,Li Xiaoxu,Wang Zhongren,Zhao Ming,Xu Xin,Han Lei,Zhao Sijun.The effect of Galectin-3 regulated Wnt signaling pathway on apoptosis of human glioma cells[J].Journal of Modern Oncology,2019,0(6):934-938. |
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| Authors: | You Yu Li Xiaoxu Wang Zhongren Zhao Ming Xu Xin Han Lei Zhao Sijun |
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| Affiliation: | Department of Neurosurgery,Affiliated Tumor Hospital of Zhengzhou University (Henan Cancer Hospital),Henan Zhengzhou 450008,China. |
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| Abstract: | Objective:To investigate the effect of Galectin-3 regulated Wnt signaling pathway on apoptosis of human glioma cells.Methods:The mRNA and protein expression of Galectin-3 in human glioma tissue were detected by RT-PCR and Western blot.Expression of Galectin-3 protein in human glioma U251,U87 and SHG-44 cells were detected by Western blot.Galectin-siRNA was transfected into human glioma U87 cells for 48 h.The expression of Galectin-3,Wnt5a,β-catenin and Cleaved Caspase3 protein and cell apoptosis were detected by Western blot and flow cytometry.Results:The expression of Galectin-3 mRNA and protein in human glioma tissue were significantly higher than those in the tumor adjacent tissues (P＜0.01).Galectin-3 protein expression in U251,U87,SHG-44 cells from high to low as U87>U251>SHG-44.U87 cells was selected as a follow-up study.Galectin-3 protein in Galectin-3-siRNA1 was lowest,selected as a follow-up study.The apoptosis rate,Cleaved Caspase3,Wnt5a,β-catenin protein expression in NC-siRNA group and the control group had no significant difference (P＞0.05).Compared with control group,the apoptosis rate in Galectin-3-siRNA group were significantly higher.Cleaved Caspase3 protein expressionwere significantly higher,and the expression of Wnt5a and β-catenin protein was significantly lower than the control group (P＜0.01).Conclusion:Silencing of Galectin-3 expression can induce apoptosis of human glioma cells,which may be related to the downregulation of Wnt signaling pathway. |
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| Keywords: | Galectin-3 Wnt signaling pathway glioma apoptosis |
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