| 酶抑制-数字图像比色法检测和鉴别食品中二硫代氨基甲酸酯、氨基甲酸酯和有机磷类农药残留 |
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| 引用本文： | 秦一帆,郭 燕,郑晓娇,白 冰,荆 旭.酶抑制-数字图像比色法检测和鉴别食品中二硫代氨基甲酸酯、氨基甲酸酯和有机磷类农药残留[J].食品安全质量检测技术,2023,14(24):44-50. |
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| 作者姓名： | 秦一帆 郭 燕 郑晓娇 白 冰 荆 旭 |
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| 作者单位： | 山西农业大学食品科学与工程学院,山西农业大学食品科学与工程学院,山西农业大学食品科学与工程学院,临汾市公安司法鉴定中心,山西农业大学食品科学与工程学院 |
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| 基金项目： | 国家自然科学基金青年科学基金项目(32202161)；山西省重点研发计划项目(202102140601018)；法庭毒物分析公安部重点实验室开放基金项目(2023FTDWFX01), |
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| 摘 要： | 建立一种绿色、高效、便捷地检测和鉴别蔬菜、水果和谷物中二硫代氨基甲酸酯、氨基甲酸酯和有机磷类农药残留的分析方法。方法 采用简单的胶带黏贴剥离方式, 结合低共熔溶剂洗脱对食品样品中的农药进行高效提取。二硫代氨基甲酸酯、氨基甲酸酯和有机磷类农药会抑制酪氨酸酶的活性, 使酪氨酸酶对酚类物质的氧化能力降低, 影响酚类物质与氯化铁生成有色物质的反应, 本方法基于此实现农药的残留分析。通过建立酪氨酸酶、酚类物质和氯化铁的比色阵列传感器, 结合主成分分析实现多种农药的区分。结果 本研究建立的酶抑制-数字图像比色法对番茄、苹果和玉米中福美锌残留在0.008~8.000 mg/kg的范围内线性良好, 检出限为0.003 mg/kg, 定量限为0.008 mg/kg, 回收率为75.2%~94.9%, 相对标准偏差小于5.0%。结论 本研究建立的酶抑制-数字图像比色法绿色环保、简单高效, 且易于推广, 适用于蔬菜、水果和谷物等食品样品中农药残留的检测, 并且在多种农药的残留分析方面有良好的应用前景。
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| 关 键 词： | 酶抑制法 数字图像比色法 低共熔溶剂 农药残留 主成分分析 |
| 收稿时间： | 2023/10/24 0:00:00 |
| 修稿时间： | 2023/12/16 0:00:00 |
Detection and identification of dithiocarbamate, carbamate, and organophosphorus pesticide residues in food by enzyme inhibition-digital image colorimetry |
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| QIN Yi-Fan,GUO Yan,ZHENG Xiao-Jiao,BAI Bing,JING Xu.Detection and identification of dithiocarbamate, carbamate, and organophosphorus pesticide residues in food by enzyme inhibition-digital image colorimetry[J].Food Safety and Quality Detection Technology,2023,14(24):44-50. |
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| Authors: | QIN Yi-Fan GUO Yan ZHENG Xiao-Jiao BAI Bing JING Xu |
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| Affiliation: | College of Food Science and Engineering, Shanxi Agricultural University,College of Food Science and Engineering, Shanxi Agricultural University,College of Food Science and Engineering, Shanxi Agricultural University,Institute of Forensic Science Public Security Bureau of Linfen,College of Food Science and Engineering, Shanxi Agricultural University |
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| Abstract: | Objective To establish a green, efficient, and convenient analytical method for the detection and identification of dithiocarbamate, carbamate, and organophosphorus pesticide residues in vegetables, fruits, and cereal. Methods The efficient extraction of pesticides was conducted through the utilization of simple adhesive tape pasting and stripping, followed by deep eutectic solvent elution. Dithiocarbamate, carbamate, and organophosphorus pesticides can inhibit the activity of tyrosinase and reduce the ability of tyrosinase to oxidize phenolics and affected the reaction between phenolics and ferric chloride to produce colored substances, which realizeed the analysis of pesticide residues. By establishing the colorimetric array sensor of tyrosinase, phenolic substances (catechol, pyrogallol, and 4-aminophenol), and ferric chloride, the principal component analysis realized the distinction of multiple pesticides (ziram, ferbam, thiram, carbofuran, carbaryl, glyphosate, and acephate). Results The enzyme inhibition combined with the digital image colorimetry method established in this study had a good linear effect on the residues of zinc in tomato, apple, and corn in the range of 0.008-8.000 mg kg-1, the limit of detection was 0.003 mg kg-1, and the limit of quantification was 0.008 mg kg-1. The recoveries ranged from 75.2% to 94.9% with relative standard deviations less than 5.0%. Conclusion The enzyme inhibition combined with the digital image colorimetry method established in this study is environmentally friendly, simple and efficient, and easy to popularize, which is suitable for the detection of pesticide residues in food samples such as vegetables, fruits, and grains, and has a good application in the analysis of multiple pesticide residues. |
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| Keywords: | enzyme inhibition digital image colorimetry deep eutectic solvent pesticide residues principal component analysis |
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