| 3种寡核苷酸探针对龈下菌斑中牙周致病菌的检测 |
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| 引用本文： | 张贤华,张斌,吴织芬.3种寡核苷酸探针对龈下菌斑中牙周致病菌的检测[J].华西口腔医学杂志,2003,21(4):287-288. |
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| 作者姓名： | 张贤华 张斌 吴织芬 |
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| 作者单位： | 610041 四川大学华西口腔医院牙周科(张贤华),第四军医大学基础部生物化学与分子生物学教研室(张 斌),第四军医大学口腔医院牙周科(吴织芬) |
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| 摘 要： | 目的 采用寡核苷酸探针研究龈下菌斑中3种牙周致病菌的分布。方法 利用3种寡核苷酸探针对 60例慢性牙周炎患者60个患病位点、10例健康人的10个健康对照位点龈下菌斑中的牙龈卟啉单胞菌、福赛类杆菌、牙密螺旋体进行检测。结果 牙周炎位点龈下菌斑中的牙龈卟啉单胞菌、福赛类杆菌、牙密螺旋体的检出率分别为91·67%,90·00%和95·67%,明显高于健康对照位点;有83·33%的牙周炎位点同时检出3种致病菌,3种细菌检出情况为两两正相关(P<0·01)。结论 牙龈卟啉单胞菌、福赛类杆菌、牙密螺旋体在慢性牙周炎患者龈下菌斑中的检出率很高,它们间可能存在相互协同致病作用。
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| 关 键 词： | 牙周炎 牙龈卟啉单胞菌 福赛类杆菌 牙密螺旋体 寡核苷酸探针 |
| 收稿时间： | 2003-08-25 |
| 修稿时间： | 2003-08-25 |
Distribution of 3 Kinds of Periodontal Pathogens in Subgingival Plaques of Patients with Chronic Periodontitis |
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| ZHANG Xianhua ,ZHANG Bin,WU Zhifen..Distribution of 3 Kinds of Periodontal Pathogens in Subgingival Plaques of Patients with Chronic Periodontitis[J].West China Journal of Stomatology,2003,21(4):287-288. |
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| Authors: | ZHANG Xianhua ZHANG Bin WU Zhifen |
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| Affiliation: | *Department ofPeriodontics,West China College ofStomatology,Sichuan Uni- versity,Chengdu610041,China |
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| Abstract: | Objective To observe the prevalence of Porphyromonas gingivalis(Pg),Treponema denticola(Td) and Bacterides forsythus(Bf) subgingivally in diseased sites of chronic periodontitis (CP) patients. Methods Samples of subgingival plaque were detected by 3 kinds of oligonucleotide DNA probe from 60 sites of CP patients and 10 healthy sites of 10 healthy people.Results The positive rates of Pg, Bf and Td in patients were 91.67%, 90.00% and 95.67% respectively; Pg, Bf and Td were detected simultaneously in 83.33% patients and Pg, Bf and Td were found to be related with each other(P<0.01).Conclusion Pg,Bf and Td were prominent periodontopathic bacteria and related each other, might exist in complexes in subgingival plaque and coaggretate together. |
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| Keywords: | periodontitis Porphyromonas gingivalis Treponema denticola Bacterides forsythus oligonucleotide DNA probe |
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