| 桂皮醛对口腔链球菌多菌种生物膜的抑制作用 |
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| 引用本文： | 张一博,张仲,董千雨,蔡锦林,周慧灵,王学润,李雨庆,王国庆.桂皮醛对口腔链球菌多菌种生物膜的抑制作用[J].现代预防医学,2023,0(7):1304-1309. |
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| 作者姓名： | 张一博 张仲 董千雨 蔡锦林 周慧灵 王学润 李雨庆 王国庆 |
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| 作者单位： | 1.四川大学华西口腔医学院口腔疾病研究国家重点实验室，国家口腔疾病临床医学研究中心，四川 成都 610041；2.四川大学华西公共卫生学院/华西第四医院，四川 成都 610041；3.四川大学华西药学院 |
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| 摘 要： | 目的 通过多种体外实验手段评价桂皮醛对三种常见口腔链球菌（变异链球菌、血链球菌和戈登链球菌）形成的多菌种生物膜的影响。方法 通过检测生长抑制作用确定桂皮醛对单菌种浮游细菌的最低抑菌浓度（minimum inhibitory concentration, MIC）；桂皮醛处理组设置 MIC、1/2 MIC和1/4 MIC三个质量浓度组；将BHIS液体培养基稀释的细菌培养液和桂皮醛溶液同时加入96孔板孔内，并放入恒温厌氧罐37℃培养24 h后，从每个孔中吸出混合液，并用PBS清洗平板，获得生物膜。通过结晶紫染色、活菌计数评估桂皮醛对单菌种和多菌种链球菌生物膜形成的影响；并通过实时荧光定量PCR测量多菌种生物膜形成相关毒力基因的表达。结果 桂皮醛对三种口腔链球菌的生长、生物膜形成以及多菌种生物膜形成均有明显抑制作用；实时荧光定量PCR结果显示桂皮醛能够抑制多菌种生物膜中gtfB、gtfC和gtfD的表达。桂皮醛还可以降低多菌种生物膜的产酸和耐酸能力。结论 一定浓度的桂皮醛对变异链球菌、血链球菌和戈登链球菌生长及生物膜形成均具有抑制作用且对多菌种生物膜形成及其毒力也有抑制作用。
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| 关 键 词： | 桂皮醛 龋病 变异链球菌 戈登链球菌 血链球菌 |
Inhibitory effect of cinnamaldehyde on multi-species biofilm of oral streptococci |
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| ZHANG Yi-bo,ZHANG Zhong,DONG Qian-yu,CAI Jin-lin,ZHOU Hui-ling,WANG Xue-run,LI Yu-qing,WANG Guo-qing.Inhibitory effect of cinnamaldehyde on multi-species biofilm of oral streptococci[J].Modern Preventive Medicine,2023,0(7):1304-1309. |
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| Authors: | ZHANG Yi-bo ZHANG Zhong DONG Qian-yu CAI Jin-lin ZHOU Hui-ling WANG Xue-run LI Yu-qing WANG Guo-qing |
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| Affiliation: | *State Key Laboratory of Oral Diseases Research, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China |
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| Abstract: | Objective To evaluate the effects of cinnamaldehyde on the multi-species biofilm formed by three common oral streptococci (Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii) through various in vitro experiments. Methods The minimum inhibitory concentration (MIC) of cinnamaldehyde to single species of planktonic bacteria was determined by detecting the growth inhibition. The cinnamaldehyde mass concentrations were set as MIC, 1/2 MIC, and 1/4 MIC for three separate treatment groups. The bacterial culture solution diluted in BHIS liquid medium and the cinnamaldehyde solution were added into the 96-well plate at the same time and then put into a constant temperature anaerobic tank at 37°C for 24 h. Biofilms were obtained after the mixed solution was sucked out and the plate was washed with PBS. The effects of cinnamon aldehyde on single-speices and multi-species Streptococcus biofilm formation were evaluated by crystal violet staining and colony-forming unit (CFU) count. The expression of virulence genes related to multi-species biofilm formation was measured by real-time fluorescent quantitative PCR. Results Cinnamaldehyde had inhibitory effects on the growth of three oral streptococci, single biofilm formation, and multi-species biofilm formation. The results of real-time PCR showed that cinnamaldehyde inhibited the expression of gtfB, gtfC, and gtfD in multi-species biofilm. This agent could also reduce the acidogenicity as well as the acid tolerance of the multi-species biofilm. Conclusion The cinnamaldehyde of certain concentration can inhibit the growth and biofilm formation of Streptococcus mutans, Streptococcus sanguinis, and Streptococcus gordonii as well as the biofilm formation and virulence of multi-species biofilm. |
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| Keywords: | Cinnamaldehyde Dental caries Streptococcus mutans Streptococcus gordonii Streptococcus sanguinis |
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