| 肝癌生长因子与血管内皮生长因子在肺癌患者血清和组织中的表达及意义 |
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| 引用本文： | 谭潇琼,周燕斌,黎银焕,黄琬玲,蔡兴东,曾庆理.肝癌生长因子与血管内皮生长因子在肺癌患者血清和组织中的表达及意义[J].中山大学学报(医学科学版),2011,32(6). |
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| 作者姓名： | 谭潇琼 周燕斌 黎银焕 黄琬玲 蔡兴东 曾庆理 |
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| 作者单位： | 中山大学附属第一医院呼吸内科,广东广州,510080 |
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| 基金项目： | 国家自然科学基金，教育部回国人员科研启动基金 |
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| 摘 要： | 【目的】探讨肝癌衍生生长因子（HDGF）和血管内皮生长因子（VEGF）表达水平与肺癌的关系。【方法】用酶联免疫吸附法（ELISA）检测健康对照组（14例）、良性肺病组（14例）、肺癌组（56例）血清中HDGF和VEGF表达水平。用免疫组织化学（IHC）的方法检测患者肺癌组织及肺癌旁正常肺组织中HDGF和VEGF的表达。【结果】肺鳞癌患者与肺腺癌患者相比，肺鳞癌患者血清HDGF水平显著增高（P<0.01）。未分化+低分化肺癌患者与中分化+高分化肺癌患者相比，未分化+低分化肺癌患者血清HDGF水平显著增高（P<0.01）；Ⅱ～Ⅳ期NSCLC患者血清HDGF和VEGF水平显著高于Ⅰ期患者（P<0.05）；有淋巴结转移者比无淋巴结转移的肺癌患者血清VEGF水平显著增高（P<0.05）；血清HDGF水平与血清VEGF水平呈显著的正相关关系（r=0.35, P<0.01）；肺癌组织中HDGF的表达水平显著高于肺癌旁正常肺组织（P<0.01）；肺癌组织中VEGF的表达水平也显著高于肺癌旁正常肺组织（P<0.05）；肺癌组织中HDGF的表达水平与VEGF的表达水平呈显著的正相关关系（r=0.75 P<0.05）。【结论】HDGF和VEGF可能与肺癌的生长、转移有关，并且两者之间可能存在着相互联系。
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| 关 键 词： | 肺癌 肝癌衍生生长因子 血管内皮生长因子 酶联免疫吸附法 免疫组织化学法 |
| 收稿时间： | 2011-05-18; |
Expression of Hepatoma-derived Growth Factor and Vascular Endothelial Growth Factor in Serum or Tissues of Patients with Lung Cancer |
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| TAN Xiao-qiong,ZHOU Yan-bin,LI Yin-huan,HUANG Wan-ling,CAI Xing-dong,ZENG Qing-li.Expression of Hepatoma-derived Growth Factor and Vascular Endothelial Growth Factor in Serum or Tissues of Patients with Lung Cancer[J].Journal of Sun Yatsen University(Medical Sciences),2011,32(6). |
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| Authors: | TAN Xiao-qiong ZHOU Yan-bin LI Yin-huan HUANG Wan-ling CAI Xing-dong ZENG Qing-li |
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| Affiliation: | TAN Xiao-qiong,ZHOU Yan-bin,LI Yin-huan,HUANG Wan-ling,CAI Xing-dong,ZENG Qing-li(Department of Respiratory Medicine,First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,China) |
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| Abstract: | 【Objective】The purpose of this study was to determine the changes and significance of hepatoma-derived growth factor (HDGF) and vascular endothelial growth factor (VEGF) in serum and lung tissue from patients with lung cancer. 【Methods】Fifty-six new inpatients diagnosed with lung cancer were enrolled as the study group, fourteen patients with benign pulmonary diseases and fourteen healthy volunteers were selected for control groups. The levels of HDGF and VEGF in serum of all subjects were detected by ELISA, and the HDGF and VEGF expression in lung cancer tissues and adjacent normal lung tissues were detected by immunohistochemical assay.【Results】 Patients with squamous cell lung cancer had higher HDGF levels in their serum than those patients with adenocarcinoma did (P<0.01). Patients with undifferentiated and poorly differentiated lung cancer had higher HDGF levels in serum than those patients with mediate and well differentiated lung cancer did (P<0.01).Patients with NSCLC in stages II-IV had higher levels of HDGF and VEGF in serum than patients with stage I disease did (P<0.05). Lung cancer patients with lymph node metastasis had higher VEGF levels in serum than patients without lymph node metastasis did (P<0.05). There was a positive correlation between VEGF and HDGF levels in serum from the patients with lung cancer (r=0.35, P<0.01). Both HDGF and VEGF expression were dramatically increased in lung tumor tissues compared with adjacent normal lung tissues (P<0.01, P<0.05).There was a positive correlation between the VEGF and HDGF levels in primary lung tumor tissues (r=0.75, P<0.05). 【Conclusions】HDGF and VEGF play an important role in tumor growth and metastasis and there is a relationship between the two factors. |
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| Keywords: | lung cancer hepatoma-derived growth factor vascular endothelial growth factor immunohistochemistry ELISA |
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