| 炎症反应中巨噬细胞的Siglec-G分子对DAMPs和PAMPs的识别及调节作用 |
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| 引用本文： | 樊亚童,郑鉴,肖俊,张学军. 炎症反应中巨噬细胞的Siglec-G分子对DAMPs和PAMPs的识别及调节作用[J]. 天津医科大学学报, 2018, 0(2): 93-96 |
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| 作者姓名： | 樊亚童 郑鉴 肖俊 张学军 |
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| 作者单位： | 天津医科大学免疫学系，天津300070 |
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| 摘 要： | 目的:探究巨噬细胞中唾液酸结合性免疫球蛋白样凝集素（Siglecs）家族的成员Siglec-G参与损伤相关分子模式（DAMPs）和病原相关分子模式（PAMPs）识别及其在炎症反应中的调节作用。方法:提取并纯化野生型小鼠腹腔巨噬细胞, 用DAMPs中的高迁移率族蛋白Bl（HMGB1）和PAMPs中的脂多糖（LPS）分别刺激24 h，Real-time PCR方法检测细胞中Siglec-G基因的表达，ELISA方法检测野生型和Siglec-G基因敲除小鼠腹腔巨噬细胞培养上清中肿瘤坏死因子α （TNF-α）和白细胞介素6（IL-6）的分泌情况。结果:HMGB1能够使巨噬细胞中Siglec-G的表达明显升高，而LPS作用后Siglec-G的表达没有明显改变，同时发现，HMGB1作用于Siglec-G敲除小鼠的巨噬细胞后，TNF-α和IL-6的分泌明显增加，而LPS刺激后炎症因子的分泌没有明显改变。结论:巨噬细胞通过表达Siglec-G来识别DAMPs并发挥免疫抑制作用，而其对于PAMPs缺乏识别及调控作用。
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| 关 键 词： | DAMPs PAMPs 巨噬细胞 Siglec-G |
Identification of DAMPs and PAMPs by Siglec-G in macrophages and its regulatory effect on DAMPs and PAMPs in inflammatory response |
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| FAN Ya-tong,ZHENG Jian,XIAO Jun,ZHANG Xue-jun. Identification of DAMPs and PAMPs by Siglec-G in macrophages and its regulatory effect on DAMPs and PAMPs in inflammatory response[J]. Journal of Tianjin Medical University, 2018, 0(2): 93-96 |
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| Authors: | FAN Ya-tong ZHENG Jian XIAO Jun ZHANG Xue-jun |
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| Affiliation: | Department of Immunology, Tianjin Medical University, Tianjin 300070, China |
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| Abstract: | Objective: To identify the PAMPs and DAMPs by Siglec-G, a member of the Sialic acid-binding immunoglobulin-like lectin (Siglecs) family, in macrophages and to explore its regulatory effect on PAMPs and DAMPs in inflammatory response. Methods: Peritoneal macrophages harvested and purified from wild type mice were stimulated with high mobility group protein Bl (HMGB1) in DAMPs and lipopolysaccharide (LPS) in PAMPs for 24 h, respectively. Real-time PCR was performed to analyze the Siglec-G mRNA expression in macrophages. ELISA was used to detect the secretion of TNF-α and IL-6 in the culture supernatants of peritoneal macrophages in wild type and Siglec-G knockout mice. Results: HMGB1 could significantly increase the expression of Siglec-G in macrophages, while the expression of Siglec-G was not altered after the stimulation of LPS. And it was found that the secretion of TNF-α and IL-6 increased significantly after HMGB1 acted on macrophages of Siglec-G knockout mice, but the secretion of inflammatory factors did not change markedly after LPS stimulation. Conclusion: Macrophages could recognize DAMPs by Siglec-G , and play a critical role in immune suppression, but it may not recognise or regulate PAMPs. |
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| Keywords: | PAMPs DAMPs macrophage Siglec-G |
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