| 大麦TIFY基因家族成员鉴定及表达分析 |
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| 引用本文： | 刘梦迪,倪守飞,王月雪,母景娇,蔡倩,赵彦宏,王艳芳.大麦TIFY基因家族成员鉴定及表达分析[J].南方农业学报,2022,53(2):417-429. |
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| 作者姓名： | 刘梦迪 倪守飞 王月雪 母景娇 蔡倩 赵彦宏 王艳芳 |
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| 作者单位： | 1 鲁东大学农学院/山东省高校作物高产抗逆分子模块育种重点实验室, 山东烟台 264025;2 鲁东大学生命与科学学院, 山东烟台 264025 |
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| 摘 要： | 【目的】对大麦TIFY基因家族成员进行鉴定及表达分析，为进一步探究TIFY基因家族在大麦生长发育与胁迫响应中的作用机理打下基础。【方法】基于TIFY家族蛋白的保守域特征，利用HMMER从大麦中鉴定TIFY基因家族成员，利用采用生物信息学软件对其理化性质、保守基序、特征结构域、顺式作用元件、基因结构、系统进化及表达模式进行预测分析。【结果】从大麦中鉴定出15个HvTIFYs基因（HvTIFY1～HvTIFY15），分布于5条染色体上，且大多数基因在染色体上成簇分布。15个HvTIFYs蛋白均具有TIFY家族蛋白的特征结构域（TIFY），根据所含保守结构域的不同，可分为ZML（4个）和JAZ亚族（11个），且亲水性蛋白（14个）和偏碱性蛋白（11个）居多，但均定位于细胞核；二级结构相似度较高，均由α-螺旋、β-转角和无规则卷曲组成，除HvTIFY7蛋白外，其余蛋白二级结构所占比排序:无规则卷曲>α-螺旋>β-转角。HvTIFYs基因结构存在明显差异，其中，JAZ亚族11个基因的内含子数为0~6； ZML亚族4个基因的内含子数为6～7个，系统发育进化树上相邻分支的基因具有较相似的基因结构。HvTIFYs基因启动子区域富含光、激素和胁迫等顺式作用元件，种类及分布均呈多样性。5个物种的79条TIFY蛋白分为4个组，恰好与TIFY家族的4个亚族对应，其中，ZML、TIFY和JAZ亚族包含单、双子叶植物的TIFY蛋白，而PPD亚族仅含有双子叶植物的TIFY蛋白。15个HvTIFYs基因在不同组织器官中的表达量存在明显差异，其中HvTIFY1、HvTIFY2和HvTIFY8基因在8个组织中的表达量均较高，HvTIFY10和HvTIFY15基因表达量中等，HvTIFY6基因表达量较低； HvTIFY11基因不表达。15个基因在根的不同组织中对盐胁迫的敏感程度不同。【结论】从大麦中鉴定出的15个HvTIFYs基因存在一定的功能分化，具有明显的组织和时空特异性，推测其在大麦逆境响应和激素调节中具有重要调控作用。
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| 关 键 词： | 大麦 TIFY基因家族 鉴定 生物信息学 表达分析 |
| 收稿时间： | 2021-11-15 |
Identification and expression analysis of TIFY gene family members in barley |
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| LIU Meng-di,NI Shou-fei,WANG Yue-xue,MU Jing-jiao,CAI Qian,ZHAO Yan-hong,WANG Yan-fang.Identification and expression analysis of TIFY gene family members in barley[J].Journal of Southern Agriculture,2022,53(2):417-429. |
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| Authors: | LIU Meng-di NI Shou-fei WANG Yue-xue MU Jing-jiao CAI Qian ZHAO Yan-hong WANG Yan-fang |
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| Affiliation: | 1 Agricultural College, Ludong University/Key Laboratory of Molecular Module Breeding for High Yield and Stress Resistance of Crops in Colleges and Universities of Shandong Province, Yantai, Shandong 264025, China;2 College of Life and Science, Ludong University, Yantai, Shandong 264025, China |
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| Abstract: | 【Objective】The identification and expression analysis of barley TIFY gene family members could lay a foundation for the further exploration and understanding of the mechanism of TIFY gene family in barley growth and development and response to stress.【Method】TIFY gene family members were identified from barley by HMMER based on their conserved domain characteristics. Their physicochemical properties,conserved motifs, characteristic domains,cisacting elements, gene structure, phylogeny and expression patterns were predicted using bioinformatics software.【Result】In this study, 15 TIFY genes(HvTIFY1-HvTIFY15) were identified in barley, which were distributed on 5 chromosomes, some of them in clusters. The 15 HvTIFY proteins had the characteristic domain(TIFY) of the TIFY family. According to their different contents of conserved domains,they could be divided into ZML(4) and JAZ(11) subgroups. Most of them were hydrophilic(14) and lightly basic(11) proteins, but all of them were located in the nucleus. With the exception of HvTIFY7,the similarity of secondary structure was high,which was proportionally composed of random coil>α-helix>β- angle. There were,however,obvious differences in the structure of HvTIFYs genes. The number of introns ranged from 0 to 6 for the 11 genes in the Jaz subfamily and from 6 to 7 for the 4 genes in the ZML subfamily. The promoter regions of HvTIFY genes were rich in cis-acting elements such as light,hormone and stress,and its species and distribution were diverse. The 79 TIFY proteins of 5 species were divided into 4 subfamilies. The ZML, TIFY and JAZ subfamilies contain TIFY proteins of monocotyledons and dicotyledons,while the PPD subfamily only contains TIFY proteins of dicotyledons. There were significant differences in the expression of the 15 HvTIFY genes in different tissues and organs, in which HvTIFY1,HvTIFY2 and HvTIFY8 genes were highly expressed in 8 tissues, HvTIFY10 and HvTIFY15 genes were moderately expressed,HvTIFY6 expression was low and HvTIFY11 expression was not detected. Different HvTIFY genes displayed different sensitivities to salt stress in different root tissues.【Conclusion】There is some functional differentiation in the 15 HvTIFY genes identified from barley with obvious tissue and spatiotemporal specificity,which are speculated to have important regulatory roles in hormone regulation and the barley response to adverse conditions. |
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