| 微小RNA - 184促进滋养层细胞凋亡而引起复发性流产的研究 |
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| 引用本文： | 梁婷婷,赵靖嵩,马成龙,杨阳,武雅婷,谢嘉渝,张慧东.微小RNA - 184促进滋养层细胞凋亡而引起复发性流产的研究[J].现代预防医学,2021,0(6):1123-1127. |
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| 作者姓名： | 梁婷婷 赵靖嵩 马成龙 杨阳 武雅婷 谢嘉渝 张慧东 |
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| 作者单位： | 四川大学华西公共卫生学院/华西第四医院，四川 成都610041 |
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| 摘 要： | 目的 探究微小RNA - 184（miR - 184）在调控女性滋养层细胞凋亡中的作用及其分子机制，为复发性流产的预防和治疗提供新思路。 方法 采用复发性流产绒毛膜组织和滋养层Swan 71细胞，采用含过表达miR - 184的质粒转染Swan 71细胞后，流式细胞仪测定细胞凋亡率，CCK8测定细胞增殖，实时荧光定量PCR和western blot法测定细胞中miR - 184、Noxa1和MCL1的表达水平。同时也在复发性流产组织中测定上述分子的表达水平。采用t检验比较两组数据的差异，采用重复测量方差分析比较两组增殖曲线。结果 与人工流产组相比，复发性流产组中miR - 184上调6.24倍（t = 3.59，P＜0.05），Noxa1上调2.19倍（t = 2.55，P＜0.05），MCL1下调80.3%（t = 2.63，P＜0.05）。与对照组相比，过表达miR - 184的滋养层细胞凋亡率增加6%（t = 4.62，P＜0.05），细胞增殖下降（F = 10.52, P＜0.05），Noxa1表达上调1.94倍（t = 7.20，P＜0.05），MCL1表达下调55.7%（t = 7.20，P＜0.05）。结论 miR - 184在复发性流产组织中高表达，其通过活化Noxa1/MCL1凋亡通路而诱导滋养层细胞凋亡，最终引发流产，为miR - 184参与调控复发性流产的发生发展提供了实验依据。
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| 关 键 词： | miR - 184 复发性流产 滋养层细胞凋亡 Noxa1/MCL1通路 |
MiR-184 promotes human trophoblastic cell apoptosis and induces recurrent miscarriage |
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| LIANG Ting-ting,ZHAO Jing-song,MA Cheng-long,YANG Yang,WU Ya-ting,XIE Jia-yu,ZHANG Hui-dong.MiR-184 promotes human trophoblastic cell apoptosis and induces recurrent miscarriage[J].Modern Preventive Medicine,2021,0(6):1123-1127. |
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| Authors: | LIANG Ting-ting ZHAO Jing-song MA Cheng-long YANG Yang WU Ya-ting XIE Jia-yu ZHANG Hui-dong |
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| Affiliation: | West China School of Public Health, Sichuan University, Chengdu, Sichuan 610041, China |
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| Abstract: | To explore whether miR-184 could promote trophoblastic cell apoptosis and to provide some insights for the prevention and treatment of recurrent miscarriage(RM). Methods The apoptosis of trophoblastic Swan 71 cells was measured by flow cytometry, and the cell proliferation was analyzed by CCK8 assays. The mRNA and protein levels of miR-184, Noxa1, and MCL1 in RM tissues and Swan71 cells were measured by RT-qPCR and Western blot. Student ’s t test and repeated measures analysis of variance were performed. Results Compared with healthy control(HC) tissues, the levels of miR-184 and Noxa1 were up-regulated 6.24-fold(t=3.59, P<0.05) and 2.19-fold(t=2.55, P<0.05), respectively, and the levels of MCL1 were down-regulated by 80.3%(t=2.63, P<0.05) in RM tissues. Compared with vector group, cell apoptosis rate was increased by 6%(t=4.62, P<0.05), cell proliferation was decreased(F=10.52, P<0.05), the levels of Noxa1 were upregulated 1.94-fold(t=7.20,P<0.05), and the levels of MCL1 were down-regulated by 55.7%(t=7.20,P<0.05) in pMDH1-PGK-GFP-miR-184 group. Conclusion Mi R-184 is up-regulated in RM tissues, which induces trophoblastic cell apoptosis by activating Noxa1/MCL1 apoptosis pathway and eventually leads to miscarriage. This work demonstrates the regulating roles of miR-184 in recurrent miscarriage. |
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| Keywords: | MiR-184 Recurrent miscarriage Human trophoblastic cell apoptosis Noxa1/MCL1 pathway |
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