| hCLP46基因在不同侵袭转移潜能乳腺癌细胞表达中的差异及其意义 |
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| 引用本文： | 姜永冬,陈艳波,何川,潘薇,庞达,张国强.hCLP46基因在不同侵袭转移潜能乳腺癌细胞表达中的差异及其意义[J].实用肿瘤学杂志,2012,26(4):336-339. |
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| 作者姓名： | 姜永冬 陈艳波 何川 潘薇 庞达 张国强 |
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| 作者单位： | 哈尔滨医科大学附属第三医院乳腺外科一病区(哈尔滨 150081) |
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| 摘 要： | 目的 探讨hCLP46基因在MCF-7和MDA-MB-231乳腺癌细胞中表达的差异性及其意义。方法 采用RT-PCR的方法检测2个细胞系(乳腺癌低度侵袭转移细胞系MCF-7、中度侵袭转移细胞系MDA-MB-231)中hCLP46 mRNA的表达差异。取MCF-7和MDA-MB-231细胞株进行培养,分别加入100 μmol/L的转化生长因子-β(TGF-β)并设对照组,培养72 h,用Western-blot方法分析P15蛋白表达。结果 RT-PCR检测hCLP46结果显示,在MCF-7和MDA-MB-231细胞系中,内参基因GAPDH的表达量相近,hCLP46基因在两个细胞系中均表达,其中MDA-MB-231细胞系中的表达高于MCF-7细胞系。两个细胞系分别加入TGF-β培养72 h后,与相应的对照细胞系相比,P15的表达均有降低,hCLP46基因高表达的MDA-MB-231细胞中P15表达较MCF-7细胞显著降低。结论 hCLP46基因过表达可能抑制TGF-β对MDA-MB-231和MCF-7乳腺癌细胞P15基因的上调,hCLP46可能在乳腺癌的发病中起到一定作用。
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| 关 键 词： | 乳腺肿瘤 hCLP46 P15 逆转录聚合酶链反应 |
| 收稿时间： | 2011-11-01 |
Differential expression and significance of hCLP46 in breast cancer cell line with different invasive and metastatic potentials |
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| JIANG Yongdong
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CHEN Yanbo
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HE Chuan
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PAN Wei
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PANG Da
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ZHANG Guoqiang.Differential expression and significance of hCLP46 in breast cancer cell line with different invasive and metastatic potentials[J].Journal of Practical Oncology,2012,26(4):336-339. |
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| Authors: | JIANG Yongdong CHEN Yanbo HE Chuan PAN Wei PANG Da ZHANG Guoqiang |
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| Affiliation: | Department of Breast Surgery,The Third Affiliated Hospital of Harbin Medical University,Harbin 150081 |
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| Abstract: | Objective To investigate hCLP46 expression and significance in two breast cancer cell lines with different invasive and metastatic potentials. Methods hCLP46 expression at mRNA level was detected by RT- PCR in two different breast cancer cell lines （MCF- 7 which was low metastatic and MDA -MB -231 which was medium metastatic）. MCF -7 and MDA -MB -231 cell lines were cultured with 100 μmol/L TGF - 13 for 72 hours respectively as the experimental group. MCF - 7 and MDA - MB - 231 cell lines were cultured without TGF - β for 72 hours as the control group. Western - blotting method was used to test the P15 expression in the two cell lines. Results RT - PCR results showed that the reference gene GAPDH expressed similarly in the two cell lines,and hCLP46 expression showed higher in MDA -MB -231 cell line than that in MCF -7 cell line. The expression of P15 in both two cell tines decreased after adding TGF - β ,compared with the correspond- ing control cell lines. The expression of P15 in MDA - MB -231 cell line was lower than that in MCF -7 cell line. Conclusion It was shown that over expression of hCLP46 may inhibit the expression of P15 inducted by TGF - β in MDA - MB - 231 and MCF - 7 cell line, hCLP46 maybe play a role in the development of breast cancer. |
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| Keywords: | Breast neoplasm hCLP46 P15 RT - PCR |
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