| 田梅,朴春姬,刘林林,杨巍,李修义.pEgr-hPTEN稳定转染联合辐射诱导人胶质瘤SHG-44细胞凋亡及Bcl-2表达下调[J].中华放射医学与防护杂志,2006,26(2):106-109 |
| pEgr-hPTEN稳定转染联合辐射诱导人胶质瘤SHG-44细胞凋亡及Bcl-2表达下调 |
| Apoptosis and down-reguled expression of Bcl-2 in SHG-44 glioma cells induced by pEgr-hPTEN stable transfection in combination with X-ray irradiation |
| 投稿时间：2005-04-23 |
| DOI： |
| 中文关键词: pEgr-hPTEN X射线 胶质瘤 细胞凋亡 Bcl-2 |
| 英文关键词:pEgr-hPTEN X-ray Glioma Apoptosis Bcl-2 |
| 基金项目:国家自然科学基金资助项目(30170290) |
|
| 摘要点击次数: 3067 |
| 全文下载次数: 1835 |
| 中文摘要: |
| 目的 探讨辐射诱导表达载体pEgr hPTEN体外稳定转染人胶质瘤SHG44细胞后联合X射线照射,诱导细胞凋亡的作用及凋亡相关蛋白Bcl2表达的变化。方法 以脂质体介导携有外源野生型PTEN基因的辐射诱导表达载体pEgr hPTEN,体外转染SHG44细胞,筛选稳定转染的细胞克隆并扩增培养;应用电子显微镜、流式细胞仪等方法,检测稳定转染联合X射线照射对胶质瘤细胞超微结构、细胞凋亡及Bcl2蛋白表达等特性的影响。结果 稳定转染细胞超微结构有明显的退行性改变,可见核内染色质趋边的类似早期凋亡的改变;稳定转染联合X射线照射可诱导肿瘤细胞凋亡,5Gy以内随吸收剂量的增加,早期凋亡细胞百分数明显增加,稳定转染不同剂量照射组早期凋亡细胞百分数分别为稳定转染0Gy假照组的1.5～2.3倍、为未转染照射组的1.9～4.4倍、为未转染0Gy假照组的3.4～5.1倍;同时稳定转染细胞Bcl2蛋白表达则呈剂量依赖性下降。结论 体外PTEN基因转染联合X射线照射可诱导肿瘤细胞凋亡明显增多,Bcl2蛋白表达下调,具有显著的肿瘤抑制作用。 |
| 英文摘要: |
| Objective To investigate the apoptotic effect and the changes of Bcl-2 protein expression of pEgr-hPTEN stable transfection in SHG-44 human glioma cells in combination with irradiation. Methods pEgr-hPTEN vector containing the exogenous wild type PTEN gene was transfected into SHG-44 cells under mediation of lipofectamine in vitro; the positive cell clones called SHG-44-hPTEN were selected and amplified by using G418. Transmission electron-microscope was used to detect the cell ultrastructural changes and flow cytometry to measure the apoptotic effect and Bcl-2 expression of SHG-44- hPTEN cells after X-ray irradiation at different doses. Results Many degenerative changes and early apoptotic changes including the chromosome condensate around the nuclear envelope were observed in SHG-44- hPTEN cells. pEgr-hPTEN stable transfection in combination with X-ray irradiation can significantly induce the apoptosis of SHG-44 cells. The percentage of early apoptosis is of SHG-44-hPTEN cells irradiated with X-rays at different doses was 1.5-2.3 times as much as that of SHG-44-hPTEN/0 Gy group, 1.9-4.4 times as much as that of SHG-44 irradiated group, and 3.4-5.1 times as much as that of SHG-44/0 Gy group. The expression of Bcl-2 proteins decreased in dose-dependent manner. Conclusion PTEN stable transfection in combination with irradiation can significantly induce the apoptosis of tumor cells and significantly down-regulate the expression of Bcl-2 protein. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |