Objective :To explore the effects of Huazhuo Jiedu Recipe(HZJD) on the proliferation and activity in rat hepatic Stellate cells(HSCs) in vitro. Method :The drug-containing serum of HZJD was prepared by feeding healthy SD rats at various doses(20,15,10 mL·kg^-1·d^-1) of HZJD, and used to incubate the HSCs cultured in vitro. The impact of drug-containing serum on HSCs proliferation was estimated by methyl thiazolyl tetrazolium(MTT)at 24,48 h and 72 h after by intervention of drug-containing serum; the content of collagen 1(COL I) was detected by enzyme linked immunosorbent assay (ELISA); the expression of transforming growth factor betal(TGF-β₁) mRNA was measured by RT-PCR. Result: The proliferation of HSCs was inhibited significantly after intervention with various dose of HZJD drug-containing serum. With a concentration-dependent mannercompared with the normal serum control group(P<0.05). The content of COL I and the expression of TGF-β₁ mRNA were degraded after intervened with HZJD drug-containing serum at various dose in HSCs (P<0.05). Conclusion: Drug-congtaining serum of HZJD could inhibit the proliferation and activity of HSCs in vitro.