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《现代肿瘤医学》[ISSN:1672-4992/CN:61-1415/R]

期数:

2021年07期

页码:

1187-1191

栏目:

论著（消化·泌尿系肿瘤）

出版日期:

2021-02-26

文章信息/Info

Title:

Expression and significance of STMN1 and UBE2C in gastric carcinoma tissues

作者:

赵 龙¹; 张惠卿²; 王 前²; 郭 星²; 张培军²

1.承德医学院，河北 承德 067000; 2.保定市第一中心医院，河北 保定 071000

Author(s):

ZHAO Long¹; ZHANG Huiqing²; WANG Qian²; GUO Xing²; ZHANG Peijun²

1.Chengde Medical College,Hebei Chengde 067000，China;2.The First Central Hospital of Baoding,Hebei Baoding 071000,China.

关键词:

STMN1; UBE2C; 胃癌; 基因表达

Keywords:

STMN1; UBE2C; gastric cancer; gene expression

分类号:

R735.2

DOI:

10.3969/j.issn.1672-4992.2021.07.018

文献标识码:

A

摘要:

目的：通过检测STMN1及UBE2C基因在胃癌组织中的表达，探索其表达与临床病理因素间的关联及两基因表达间的关联。方法：采用免疫组化的方法检测180例胃癌病理标本及对应180例正常胃部黏膜标本中STMN1及UBE2C基因的表达情况。结果：STMN1在胃癌组织中的阳性表达率为85.6%，而在正常组织中阳性表达率为26.1%(P＜0.05)，STMN1的表达与患者性别、年龄及肿瘤分化程度无明显相关性，而与TNM分期、淋巴结转移及Lauren分型明显相关（P＜0.05)。UBE2C在胃癌组织中的阳性表达率为73.3%，而在正常组织中阳性表达仅为24.4%(P＜0.05)，UBE2C的表达与患者性别、年龄及肿瘤分化程度均无显著相关，而与淋巴结转移、TNM分期及Lauren分型关联性显著(P＜0.05)。Logistic多因素分析表明，淋巴结转移、Lauren分型、TNM分期与STMN1及UBE2C表达相关(P＜0.05)。STMN1与UBE2C基因表达呈正相关(r=0.288，P＜0.05)。结论：STMN1的表达与胃癌的产生、进展、浸润及转移密切相关，UBE2C可能通过PI3K/Akt信号通道调节STMN1。

Abstract:

Objective:To detect the expression of STMN1 and UBE2C genes in gastric cancer tissues and to explore the relationship between them and clinicopathological factors.Methods:The expression of STMN1 and UBE2C genes was detected by immunohistochemical method in 180 cases of gastric cancer tissues and 180 cases of normal gastric mucosa tissues.Results:The positive expression rate of STMN1 in gastric cancer tissues was 85.6%,while that in normal tissues was 26.1%.There was no significant correlation between the expression of STMN1 and gender,age and tumor differentiation.It was significantly correlated with TNM stage,lymph node metastasis and Lauren classification.The positive expression rate of UBE2C in gastric cancer tissues was 73.3%,while that in normal tissues was only 24.4%（P＜0.05).The expression of UBE2C was not significantly correlated with gender,age and tumor differentiation,but significantly correlated with lymph node metastasis,TNM stage and Lauren classification（P＜0.05).Logistic multivariate analysis showed that lymph node metastasis,Lauren classification and TNM stage were correlated with the expression of STMN1 and UBE2C in gastric cancer tissues.There was a positive correlation between STMN1 and UBE2C gene expression.Conclusion:The expression of STMN1 is closely related to the occurrence,progression,invasion and metastasis of gastric cancer,and UBE2C may regulate STMN1 through PI3K/Akt signal pathway.

参考文献/References

[1]RAWLA P,BARSOUK A.Epidemiology of gastric cancer:global trends,risk factors and prevention［J］.Przeglad Gastroenterologiczny,2019,14(1):26-38.
[2]DIGKLIA A.Advanced gastric cancer:Current treatment landscape and future perspectives［J］.World Journal of Gastroenterology,2016,22(8):2403-2414.
[3]KOUZU Y,UZAWA K,KOIKE H,et al.Overexpression of stathmin in oral squamous-cell carcinoma:correlation with tumour progression and poor prognosis［J］.British Journal of Cancer,2006,94(5):717-723.
[4]GUPTA KK,LI C,DUAN A,et al.Mechanism for the catastrophe-promoting activity of the microtubule destabilizer p18/stathmin［J］.Proceedings of the National Academy of Sciences,2013,110(51):20449-20454.
[5]DASTSOOZ H,CEREDA M,DONNA D,et al.A comprehensive bioinformatics analysis of UBE2C in cancers［J］.International Journal of Molecular Sciences,2019,20(9):2228-2247.
[6]敖先伟,张涛,黄李冰雪,等.曲妥珠单抗在人表皮生长因子受体2阳性胃癌治疗中的应用及其耐药机制研究进展［J］.广西医学,2020,42(05):617-621. AO XW,ZHANG T,HUANG-LI BX,et al.Application of trastuzumab in the treatment of human epidermal growth factor receptor 2 positive gastric cancer and its drug resistance mechanism［J］.Guangxi Medicine,2020,42（05):617-621.
[7]周东辉,吴璇,钭赉振,等.晚期胃癌分子靶向治疗和免疫治疗的研究进展［J］.浙江医学,2020,42(03):203-208. ZHOU DH,WU X,LUO JZ,et al.Advances in molecular targeted therapy and immunotherapy for advanced gastric cancer ［J］.Zhejiang Medicine,2020,42（03):203-208.
[8]张佳佳,喻鑫,周波,等.SNAP29调节胃癌细胞顺铂耐药性的研究［J］.安徽医科大学学报,2020,55(08):1189-1193. ZHANG JJ,YU X,ZHOU B,et al.SNAP29 regulates cisplatin resistance in gastric cancer cells ［J］.Journal of Anhui Medical University,2020,55（08):1189-1193.
[9]YURONG L,BIAOXUE R,WEI L,et al.Stathmin overexpression is associated with growth,invasion and metastasis of lung adenocarcinoma［J］.Oncotarget,2017,8(16):26000-26012.
[10]崔青荣，杨秦敏，李彬，等.siRNA沉默STMN1基因抑制肺癌细胞增殖与侵袭的机制探讨［J］.现代肿瘤医学，2018,26（14）：2143-2146. CUI QR,YANG QM,LI B,et al.The mechanism researches of silencing of STMN1 gene by siRNA inhibited the proliferation and invasion of lung cancer cells［J］.Modern Oncology，2018,26（14）：2143-2146.
[11]OBAYASHI S,HORIGUCHI J,HIGUCHI T,et al.Stathmin1 expression is associated with aggressive phenotypes and cancer stem cell marker expression in breast cancer patients［J］.International Journal of Oncology,2017,51(3):781-790.
[12]OGINO S,NOSHO K,BABA Y,et al.A cohort study of STMN1 expression in colorectal cancer:Body mass index and prognosis［J］.The American Journal of Gastroenterology,2009,104(8):2047-2056.
[13]KE B,GUO X,LI N,et al.Clinical significance of Stathmin1 expression and epithelial-mesenchymal transition in curatively resected gastric cancer［J］.Molecular and Clinical Oncology,2019,10(2):214-222.
[14]KANG W,TONG JHM,CHAN AWH,et al.Stathmin1 plays oncogenic role and is a target of microRNA-223 in gastric cancer［J］.PLoS One,2012,7(3):e33919.
[15]BAI T,YOKOBORI T,ALTAN B,et al.High STMN1 level is associated with chemo-resistance and poor prognosis in gastric cancer patients［J］.British Journal of Cancer,2017,116(9):1177-1185.
[16]马子涵,贾西云,徐倩,等.胃癌组织中AKT1与STMN1表达的关系及其临床意义［J］.肿瘤基础与临床,2019,32(6):471-474. MA ZH,JIA XY,XU Q,et al.Relationship between AKT1 and STMN1 expression in gastric cancer and its clinical significance［J］.Basic and Clinical Oncology,2019,32（6):471-474.
[17]KARST AM,LEVANON K,DURAISAMY S,et al.Stathmin 1,a marker of PI3K pathway activation and regulator of microtubule dynamics,is expressed in early pelvic serous carcinomas［J］.Gynecologic Oncology,2011,123(1):5-12.
[18]PERROTTA I,BRUNO L,MALTESE L,et al.Immunohistochemical analysis of the uiquitin-conjugating enzyme UbcH10 in lung cancer［J］.Journal of Histochemistry & Cytochemistry,2012,60(5):359-365.
[19]MO C,GAO L,ZHU X,et al.The clinicopathological significance of UBE2C in breast cancer:a study based on immunohistochemistry,microarray and RNA-sequencing data［J］.Cancer Cell International,2017,17(1):83-100.
[20]XIONG Y,LU J,FANG Q,et al.UBE2C functions as a potential oncogene by enhancing cell proliferation,migration,invasion,and drug resistance in hepatocellular carcinoma cells［J］.Bioscience Reports,2019,39(4):2384-2392.
[21]SHEN Z,JIANG X,ZENG C,et al.High expression of ubiquitin-conjugating enzyme 2C（UBE2C) correlates with nasopharyngeal carcinoma progression［J］.BMC Cancer,2013,13(1):192-202.
[22]FUJITA T,IKEDA H,TAIRA N,et al.Overexpression of UbcH10 alternates the cell cycle profile and accelerate the tumor proliferation in colon cancer［J］.BMC Cancer,2009,9(1):87-97.
[23]LIN J,RAOOF D A,WANG Z,et al.Expression and effect of inhibition of the ubiquitin-conjugating enzyme E2C on esophageal adenocarcinoma［J］.Neoplasia,2006,8(12):1062-1071.
[24]MA R,KANG X,ZHANG G,et al.High expression of UBE2C is associated with the aggressive progression and poor outcome of malignant glioma［J］.Oncology Letters,2016,11(3):2300-2304.
[25]BAJAJ S,ALAM SK,ROY KS,et al.E2 ubiquitin-conjugating enzyme,UBE2C gene,is reciprocally regulated by wild-type and gain-of-function mutant p53［J］.Journal of Biological Chemistry,2016,291(27):14231-14247.
[26]BAVI P,UDDIN S,AHMED M,et al.Bortezomib stabilizes mitotic Cyclins and prevents cell cycle progression via inhibition of UBE2C in colorectal carcinoma［J］.The American Journal of Pathology,2011,178(5):2109-2120.
[27]IANCU C,MISTRY SJ,ARKIN S,et al.Taxol and anti-stathmin therapy:a synergistic combination that targets the mitotic spindle［J］.Cancer Research,2000,60(13):3537-3543.
[28]MLAK R,KRAWCZYK P,CIESIELKA M,et al.Predictive value of STMN1 gene promoter polymorphism（-2166T>C) in patients with advanced NSCLC treated with the combination of platinum compounds and vinorelbine［J］.Cancer Chemotherapy and Pharmacology,2015,76(3):621-629.
[29]WANG R,SONG Y,LIU X,et al.UBE2C induces EMT through Wnt/β-catenin and PI3K/Akt signaling pathways by regulating phosphorylation levels of Aurora-A［J］.International Journal of Oncology,2017,50(4):1116-1126.

备注/Memo

备注/Memo:

National Natural Science Foundation of China(No.81773178）；国家自然科学基金面上项目(编号：81773178)

常用功能

更新日期/Last Update: 2021-02-26