盐酸罗哌卡因对胃癌MGC-803细胞增殖和凋亡的作用
《现代肿瘤医学》[ISSN:1672-4992/CN:61-1415/R]
- 期数:
- 2020年08期
- 页码:
- 1261-1265
- 栏目:
- 论著（基础研究）
- 出版日期:
- 2020-03-16
文章信息/Info
- Title:
- Effect of ropivacaine on proliferation and apoptosis of gastric cancer MGC-803 cells
- Author(s):
- Zhu Jing; Zhang Shiping; Zhang Wenjuan; Li Xin; Song Yulong
- Department of Anaesthesiology,Shaanxi Provincial People's Hospital,Shaanxi Xi'an 710068,China.
- 关键词:
- 盐酸罗哌卡因; 胃癌MGC-803细胞; 细胞增殖; 凋亡
- 分类号:
- R735.2
- DOI:
- 10.3969/j.issn.1672-4992.2020.08.005
- 文献标识码:
- A
- 摘要:
- 目的:探讨盐酸罗哌卡因对人胃癌MGC-803细胞增殖和凋亡的影响。方法:细胞计数试剂盒（CCK-8）检测盐酸罗哌卡因对MGC-803细胞增殖能力的影响，并确定盐酸罗哌卡因的用药浓度，流式细胞术检测盐酸罗哌卡因对MGC-803细胞周期的影响，Annexin V-FITC/PI法检测盐酸罗哌卡因对MGC-803细胞凋亡的影响，蛋白免疫印迹法（Western blot）检测盐酸罗哌卡因对MGC-803细胞中B细胞淋巴瘤/白血病-2（Bcl-2）、Bcl-2相关X蛋白（Bax）和剪切的含半胱氨酸的天冬氨酸蛋白水解酶3（Cleaved Caspase-3）蛋白表达水平。结果:随着盐酸罗哌卡因用药浓度的升高，MGC-803细胞增殖能力逐渐降低，根据CCK-8实验结果分别筛选出浓度为10、50 μg/ml和100 μg/ml的盐酸罗哌卡因用于后续实验。盐酸罗哌卡因能够明显阻滞细胞周期于G2期，诱导细胞凋亡，抑制Bcl-2蛋白表达，促进Bax和Cleaved Caspase-3蛋白表达。结论:盐酸罗哌卡因能够抑制胃癌MGC-803细胞增殖，阻碍MGC-803细胞周期进程，诱导细胞凋亡，该过程可能与下调Bcl-2蛋白表达，上调Bax和Cleaved Caspase-3蛋白表达有关。
- Abstract:
- Objective:To investigate the effect of ropivacaine on proliferation and apoptosis of human gastric cancer MGC-803 cells.Methods:Cell counting kit (CCK-8) was used to detect the effect of ropivacaine on the proliferation of MGC-803 cells,and the concentration of ropivacaine was determined.Flow cytometry was used to detect ropivacaine on the cell cycle of MGC-803.The effect of tranexaine on apoptosis of MGC-803 cells was detected by Annexin V-FITC/PI method.Western blot was used to detect Bcl-2,Bax and Cleaved Caspase-3 protein expression levels.Results:With the increase of ropivacaine concentration,the proliferation of MGC-803 cells gradually decreased.According to the results of CCK-8,the concentrations of ropivacaine at 10,50 μg/ml and 100 μg/ml were screened for subsequent experiments.Ropivacaine can significantly block the cell cycle in G2 phase,induce apoptosis,inhibit Bcl-2 protein expression,and promote the expression of Bax and Cleaved Caspase-3 protein.Conclusion:Ropivacaine can inhibit the proliferation of gastric cancer MGC-803 cells,block the cell cycle progression of MGC-803,and induce apoptosis.This process may be related to down-regulating Bcl-2 protein expression and up-regulating Bax and Cleaved Caspase-3 protein expression.
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备注/Memo
- 备注/Memo:
- 陕西省自然科学基础研究计划项目(编号：2018JM7121)
