广东工业大学学报 ›› 2021, Vol. 38 ›› Issue (02): 94-98.doi: 10.12052/gdutxb.200059

• 综合研究 • 上一篇    下一篇

银耳多糖超声波提取工艺优化及抗BV2细胞炎症的作用研究

谢玲娜1, 韩萍2, 杜志云1   

  1. 1. 广东工业大学 生物医药学院, 广东 广州 510006;
    2. 佛山市康伲爱伦生物技术有限公司, 广东 佛山 528225
  • 收稿日期:2020-04-10 出版日期:2021-03-10 发布日期:2021-01-13
  • 通信作者: 杜志云(1969-),男,教授,研究生导师,主要研究方向为天然药物化学,E-mail:zhiyundu@gdut.edu.cn E-mail:zhiyundu@gdut.edu.cn
  • 作者简介:谢玲娜(1994-)，女，硕士研究生，主要研究方向为天然药物化学
  • 基金资助:
    国家自然科学基金资助项目(21272043)

An Optimization of Ultrasonic Extraction Process of Tremella Polysaccharides and its Anti-inflammation Effect on BV2 cell

Xie Ling-na1, Hang Ping2, Du Zhi-yun1   

  1. 1. School of Biomedicine and Pharmaceutical Science, Guangdong University of Technology, Guangzhou 510006, China;
    2. Foshan Allan Conney Biotechnology Co., Ltd, Foshan 528225, China
  • Received:2020-04-10 Online:2021-03-10 Published:2021-01-13

HTML

PDF (PC)

2637

摘要： 以银耳为原料, 采用超声波辅助热水浸提法提取银耳多糖, 以多糖提取率为指标, 以超声波功率、提取料液比、提取温度和提取时间为影响因素, 设计单因素试验, 并通过正交试验得到较佳提取工艺。通过用TRPV1(Transient Receptor Potential Vanilloid1)试剂盒检测了小胶质细胞TRPV1的释放水平, 与脂多糖(Lipopolysaccharide, LPS)对照组相比, 不同质量浓度的银耳多糖组(62.5, 250, 1000 μg/mL)均能不同程度地抑制TRPV1的释放量。说明了银耳多糖对于BV2小胶质细胞炎症具有良好的抑制作用。

关键词: 银耳多糖, 超声波, 热水浸提, 正交试验, 神经炎症, 辣椒素受体1

Abstract: In this study, the tremella polysaccharide was extracted by ultrasonic-assisted hot-water extraction from Tremellafuciformis. The polysaccharide extraction rate was used as an indicator. The ultrasonic frequency, the ratio of extraction material to liquid, the extraction temperature, and the extraction time were the influencing factors. A single factor experiment was designed. A better extraction process was obtained through orthogonal experiments. By using the TRPV1 kit to detect the release level of microglial TRPV1, compared with the LPS control group, different concentrations of the Tremella polysaccharide group (62.5, 250, 1000 μg/mL) can be different to varying degrees Inhibition of TRPV1 release. It shows that Tremella polysaccharide has a good inhibitory effect on BV2 microglia inflammation.

Key words: tremella polysaccharide, ultrasound, hot-water extraction, orthogonal test, neuroinflammation, Transient Receptor Potential Vanilloid1(TRPV 1)

中图分类号: 

  • Q946.3
[1] WU Y J, WEI Z X, ZHANG F M, et al. Structure, bioactivities and applications of the polysaccharides from tremella fuciformis mushroom: a review [J]. International Journal of Biological Macromolecules, 2019, 121: 1005-1010.
[2] 李燕, 蔡东联, 胡同杰, 等. 银耳多糖对实验性衰老小鼠的保护作用[J]. 第二军医大学学报, 2004(10): 1104-1107.
LI Y, CAI D L, HU T J, et al. Protective effect of tremella polysaccharides on experimental aging mouse model [J]. Academic Journal of Second Military Medical University, 2004(10): 1104-1107.
[3] CHEN B. Optimization of extraction of tremella fuciformis polysaccharides and its antioxidant and antitumor activities in vitro [J]. Carbohydrate Polymers, 2010, 81(2): 420-424.
[4] LI H, LEE H S, KIM S H, et al. Antioxidant and anti-inflammatory activities of methanol extracts of tremella fuciformis and its major phenolic acids [J]. Journal of Food Science, 2014, 79(4): C460-C468.
[5] HANG Z, WANG X, ZHAO M, et al. Free-radical degradation by Fe2+/Vc/H2O2 and antioxidant activity of polysaccharide from tremella fuciformis [J]. Carbohydrate Polymers, 2014, 112: 578-582.
[6] 庞良芳. 银耳孢糖提高肝癌化疗患者生存质量的研究[J]. 湖北中医药大学学报, 2014, 16(4): 85-86.
PANG L F. Clinical study about parenteral solution of tremella polysaccharide improving quality of life of patients with liver cancer during chemotherapy [J]. Journal of Hubei University of Chinese Medicine, 2014, 16(4): 85-86.
[7] UKAI S, KIHO T, HARA C, et al. Polysaccharides in fungi. XIV. anti-inflammatory effect of the polysaccharides from the fruit bodies of several fungi [J]. Pubmed, 1983, 6(12): 983-990.
[8] SHEN T, DUAN C, CHEN B, et al. Tremella fuciformis polysaccharide suppresses hydrogen peroxide-triggered injury of human skin fibroblasts via upregulation of SIRT1 [J]. Molecular Medicine Reports, 2017, 16(2): 1340-1346.
[9] BACH E E, COSTA S G, OLIVEIRA H A, et al. Use of polysaccharide extracted from tremella fuciformis berk for control diabetes induced in rats [J]. Thoracic & Cardiovascular Surgeon, 2015, 27(7): 2103-2105.
[10] 张小飞, 果秋婷, 孙静. Box-Benhnken响应面法优化超声波辅助提取银耳多糖工艺的研究[J]. 中国药师, 2019(6): 1055-1058.
ZHANG X F, GUO Q T, SUN J. Optimization of ultrasonic-assisted extraction process of tremella polysaccharides based on Box-Benhnken response surface methodology [J]. China Pharmacist, 2019(6): 1055-1058.
[11] 刘蔷, 刘磊, 康传利, 等. 银耳多糖的制备工艺及研究进展[J]. 生物产业技术, 2019(5): 58-63.
LIU Q, LIU L, KANG C L, et al. Preparation and research progress of tremella polysaccharides [J]. Biotechnology & Business, 2019(5): 58-63.
[12] 段超, 王昌涛. 银耳多糖提取工艺优化及对人体细胞毒性检测[J]. 日用化学工业, 2016, 46282(2): 51-55.
DUAN C, WANG C T. Optimization of extracting Tremella fuciformis polysaccharide and detection of its toxicity to cells of human body [J]. China Surfactant Detergent & Cosmetics, 2016, 46282(2): 51-55.
[13] 鲍会梅. 酶解法提取银耳多糖的工艺及稳定性研究[J]. 食品研究与开发, 2015(11): 17-21.
BAO H M. Study on extraction technology and stability of tremella polysaccharide by enzymatic hydrolysis [J]. Food Research and Development, 2015(11): 17-21.
[14] 吴琼, 郑成, 宁正祥, 等. 碱溶性银耳粗多糖的提取及其清除自由基作用的研究[J]. 食品科学, 2007(6): 153-155.
WU Q, ZHENG C, NING Z X, et al. Study on extraction and antioxidant effects of crude alkali-soluble tremella polysaccharide [J]. Food Science, 2007(6): 153-155.
[15] CHEN Y, ZHAO L, LIU B, et al. Application of response surface methodology to optimize microwave-assisted extraction of polysaccharide from tremella [J]. Physics Procedia, 2012, 24(part-PA): 429-433.
[16] YANG L, SUI X, QI B, et al. Optimization of ethanol-ultrasound-assisted destabilization of a cream recovered from enzymatic extraction of soybean oil [J]. Journal of the American Oil Chemists Society, 2019, 91(1): 159-168.
[17] 张卫丽, 韩雅莉. 响应面法优化马尾松松针中总黄酮提取工艺[J]. 广东工业大学学报, 2013, 30(1): 115-119, 124.
ZHANG W L, HAN Y L. The extraction process of pinus massoniana optimized by response surface [J]. Journal of Guangdong University of Technology, 2013, 30(1): 115-119, 124.
[18] 张倩茹, 凌蕾, 何芋岐, 等. 苯酚硫酸法测定苗药艾纳香中多糖含量[J]. 中国民族民间医药, 2019, 28(14): 30-32.
ZHANG Q R, LING L, HE Y Q, et al. Determination of polysaccharide content of blumea balsamifera by phenol sulfuric method [J]. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2019, 28(14): 30-32.
[19] 杨勇杰, 姜瑞芝, 陈英红, 等. 苯酚硫酸法测定杂多糖含量的研究[J]. 中成药, 2005, 27(6): 706-708.
YANG Y J, JIANG R Z, CHEN Y H, et al. Determination of sugars in heteropolysaccharide by phenol-sulfuric acid method [J]. Chinese Traditional Patent Medicine, 2005, 27(6): 706-708.
[1] 唐超兰, 谢义. 6061铝合金铣削工艺参数多目标优化[J]. 广东工业大学学报, 2020, 37(05): 87-93.
[2] 臧小豪, 刘起发, 胡蒙蒙, 常媛媛, 肖清炜, 周渭. 靶向TSPO的神经炎症成像分子探针[J]. 广东工业大学学报, 2020, 37(04): 91-97.
[3] 裴高科, 高军礼, 陶地状, 刘海杰, 马锦坚. 于DSP的超声波电机驱动器设计[J]. 广东工业大学学报, 2015, 32(3): 97-101.
[4] 张敏, 韩雅莉. 薇甘菊叶的总黄酮提取及抑菌活性研究[J]. 广东工业大学学报, 2014, 31(2): 133-138.
[5] 张卫丽，韩雅莉. 响应面法优化马尾松松针中总黄酮提取工艺[J]. 广东工业大学学报, 2013, 30(1): 115-119.
[6] 张春华，谢永军，周政毅，吴成达，周振扬. 公共场所人数检测统计系统的设计[J]. 广东工业大学学报, 2012, 29(3): 63-67.
[7] 袁国正. 复方中草药在超声波药物耦合剂中的抗菌效果[J]. 广东工业大学学报, 2011, 28(4): 74-78.
[8] 毛凌波； 张仁元； 柯秀芳； 庞晋山； . 分散方式对纳米炭黑分散稳定性的影响[J]. 广东工业大学学报, 2009, 26(3): 13-16.
[9] 董松；. 浅谈钢管混凝土柱柱芯混凝土质量检测[J]. 广东工业大学学报, 2009, 26(3): 31-33.
[10] 吴增伟； 徐天平； . 圆环式径向换能器辐射声场的研究[J]. 广东工业大学学报, 2007, 24(03): 103-.
[11] 陈海燕； 李卫民； . 铜钎焊剂的成分优化与制备[J]. 广东工业大学学报, 2006, 23(1): 60-63.
[12] 刘石生； 丘泰球； 蔡纯； 韩松涛； . 超声对壳聚糖降解作用的研究[J]. 广东工业大学学报, 2002, 19(3): 83-86.
[13] 刘勇健； 沈军； 张建龙； . 废泥浆固液分离的试验研究[J]. 广东工业大学学报, 2000, 17(2): 53-56.
[14] 张永俊； 刘晓宁； 邹君泰； 陆友慈； . 超声波色带焊接机气动工作台设计[J]. 广东工业大学学报, 2000, 17(1): 1-4.
[15] 关燕琼； 杨辉荣； 陈文庆； 李峰； 廖志云； . 扑热息痛合成工艺的研究[J]. 广东工业大学学报, 1997, 14(2): 43-46.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2017 《广东工业大学学报》编辑部
地址：广州市东风东路729号广东工业大学 邮编：510090
电话：020-37626653,020-37626139,020-37626396
邮箱：xbzrb@gdut.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发