Sexual maturity is a critical step to breakthrough of controlled reproduction of cultured Chinese sturgeon(). The suitable breeding management strategy will be beneficial to promoting the maturation progress of cultured Chinese sturgeon and to synchronous spawning of female and male. In this study, we observed the gonadal development of 250 cultured Chinese sturgeon (ages: 12–15 years old, total length: 175–286 cm, body weight: 34.6–125.3 kg) under different water temperature profiles(natural water temperature or near-formula feed, live fish or iced fish+formula feed, live fish or iced fishThe result showed that gonadal development could reach stage III under natural water temperature or a near-The gonadal development could reach stage III or IV in Chinese sturgeons fed with different types of diets, however the feed added live or iced fish is better for development from stage II to IV than the feeds with only single source. Controlled propagation was then successfully conducted with one female (12 years old with 57 kg body weight) and one male(14 years old with 64 kg body weight). A total of 62 500 eggs and 2 850 mL milt were obtained and 23 000 hatching-out The average fertilized rate was 60.1% with the range from 36.3% to 80.4%. The elementary research on breeding regulations of brood stocks will contribute to further large-scale production of cultured Chinese sturgeon.