目的 对台湾拟金发藓Polytrichastrum formosum全草的化学成分及其细胞毒活性开展研究，为其进一步开发提供科学依据。方法 采用硅胶、Sephadex LH-20凝胶、MCI-Gel树脂柱色谱、半制备型RP-HPLC等多种色谱技术进行分离纯化，运用波谱方法对所分离的化合物进行结构鉴定，采用MTT法测试化合物对人肝癌HepG2细胞和人胰腺癌PANC-1细胞的细胞毒活性。结果 从台湾拟金发藓植物中分离得到4个化合物，分别鉴定为2′′-(1-苯甲酰基-2-羟基-4-苯氧基)-N-(2′′′-羟基-乙基)-乙酰胺（1）、(2-羟基-4-甲氧基-苯基)-苯基-甲酮（2）、(2,4-二甲氧基-苯基)-苯基-甲酮（3）、(2,4-二羟基-苯基)-苯基-甲酮（4）；部分化合物显示出了一定的细胞毒活性。结论 化合物1为新化合物，命名为金发藓酮B；化合物2～4为新天然产物，首次从金发藓科植物中分离得到；化合物1对人肝癌HepG2细胞具有较强的细胞毒活性，其半数抑制浓度（median inhibition concentration，IC₅₀）值为38.14 μmol/L。

Objective To investigate the chemical constituents and cytotoxic activity of the whole plant of Polytrichastrum formosum, and provide scientific basis for its further development. Methods Various chromatographic techniques such as silica gel, Sephadex LH-20 gel, MCI-Gel resin column chromatography and semi-preparative RP-HPLC were used for separation and purification. Their structures were elucidated by spectroscopic data analyses. The cytotoxicity of compounds for HepG2 and PANC-1 cells line was also evaluated by using the MTT method. Results Four compounds were isolated and identified as 2′′-(1-benzoyl-2-hydroxy-4-phenoxy)-N-(2′′′-hydroxy-ethyl)-acetamide (1), (2-hydroxy-4-methoxy-phenyl)-phenyl-methanone (2), (2,4-dimethoxy-phenyl)-phenyl-methanone (3), and (2,4-dihydroxy-phenyl)-phenyl-methanone (4). Some compounds showed certain cytotoxic activity. Conclusion Compound 1 is a new compound named pogonatone B; Compounds 2—4 are new natural products, and isolated from Polytrichaceae species for the first time; Compound 1 also displays the high cytotoxicity on HepG2 cell with IC₅₀ values of 38.14 μmol/L.

R284.1

国家自然科学基金资助项目（31070184）；河北中医学院博士科研基金资助项目（BSZ2021020）；全国中药特色技术传承人才培训项目（T20194828003）