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| 壮药红鱼眼黄酮类化合物的提取及其抗氧化活性研究 |
| Studies on Extraction and Antioxidant Activities of Flavonoids in Phyllanthus Reticulatus |
| 投稿时间：2013-02-17 修订日期：2013-04-08 |
| DOI： |
| 中文关键词: 红鱼眼 黄酮 抗氧化活性 |
| 英文关键词:Phyllanthus reticulatus Flavonoids Antioxidant activity |
| 基金项目:广西壮族自治区科技厅资助项目（编号：09321005） |
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| 中文摘要: |
| 摘 要 目的: 研究壮药红鱼眼黄酮类化合物的提取工艺及其抗氧化活性。方法: 用乙醇回流提取法通过正交设计试验确定黄酮提取工艺的最佳条件，用氧化法测定其清除超氧自由基和DPPH· 自由基的能力。结果:红鱼眼黄酮提取的最佳条件为：8倍量60%乙醇提取3次，每次1 h。所得黄酮在一定浓度范围内具有较强的清除自由基和抗氧化能力。结论:优选的提取工艺效率高，所制备的黄酮有较强的清除超氧自由基和DPPH· 自由基活性，具有很好的抗自氧化效果，是一种较好的天然抗氧化剂。 |
| 英文摘要: |
| ABSTRACT Objective: To study the extraction technology and antioxidant activity of flavonoids in Phyllanthus reticulatus. Method:The optimal extraction technology of flavonoids was screened by orthogonal design, and the clearance of superoxide radical and DPPH· were detected by oxidation method.Result:The optimal extraction conditions were as follows: adding 8 fold of 60％ ethanol and extracting 3 times with 60 min for each. The obtained flavonoids showed significant radical scavenging and antioxidant activity within the experimental concentration range. Conclusion:The optimal extraction technology exhibits higher extraction efficiency, and the flavonoids show promising radical scavenging and antioxidant activity. The obtained flavonoids can be used as promising natural antioxidants. |
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