Objective To evaluate clinical efficacy and safety of endoscopic application of detachable titanium clip and rubber-band traction in difficult biliary cannulation patients with the papilla of vater in the diverticula during endoscopic retrograde cholangiopancreatography (ERCP).Methods The clinical data of 869 patients with biliary tract diseases who underwent ERCP from March 2020 to October 2021 were retrospectively analyzed. 127 patients with difficult bile ducts combined with the papilla of vater in the diverticula who failed in the intial routine intubation were selected. Subsequently, these patients who used the removable detachable titanium clip combined with rubber-band traction to re-intubation of bile duct were analyzed. The main observation indicators included success rate of bile duct intubation, operation time and post-operative complications.Results There were 127 (14.61%, 127/869) patients with difficult biliary cannulation, and 14 patients with difficult biliary cannulation and the papilla of vater in the diverticula were still unable to enter the bile duct after routine intubation. and then they were intubated again with the auxiliary technology of detachable titanium clip combined with rubber-band traction, and the 13 patients (92.86%) were successfully. The mean operation time was (6.23±2.87) min, post-ERCP hyperamylasemia occurred in 2 patients and mild pancreatitis occurred in 1 patients, without other complications.Conclusion Detachable titanium clip and rubber-band traction is a safe and feasible method for difficult biliary cannulation patients with the papilla of vater in the diverticula during ERCP. It is worthy of clinical application.