| 不同产地和不同品种枸杞子中多糖成分测定 |
| 投稿时间：2021-11-03 点此下载全文
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| 引用本文：王莹,时锐,张南平,辜冬琳,昝珂,刘越,金红宇,马双成.不同产地和不同品种枸杞子中多糖成分测定[J].中国现代中药,2022,24(6):996-1002 |
| DOI：10.13313/j.issn.1673-4890.20211103001 |
| 摘要点击次数: 358 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 王莹 |
WANG Ying |
中国食品药品检定研究院，北京 102629 |
National Institutes for Food and Drug Control, Beijing 102629, China |
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| 时锐 |
SHI Rui |
北京中医药大学，北京 100029 |
Beijing University of Chinese Medicine, Beijing 100029, China |
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| 张南平 |
ZHANG Nan-ping |
中国食品药品检定研究院，北京 102629 |
National Institutes for Food and Drug Control, Beijing 102629, China |
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| 辜冬琳 |
GU Dong-lin |
中国食品药品检定研究院，北京 102629 |
National Institutes for Food and Drug Control, Beijing 102629, China |
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| 昝珂 |
ZAN Ke |
中国食品药品检定研究院，北京 102629 |
National Institutes for Food and Drug Control, Beijing 102629, China |
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| 刘越* |
LIU Yue |
北京中医药大学，北京 100029 |
Beijing University of Chinese Medicine, Beijing 100029, China |
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| 金红宇 |
JIN Hong-yu |
中国食品药品检定研究院，北京 102629 |
National Institutes for Food and Drug Control, Beijing 102629, China |
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| 马双成 |
MA Shuang-cheng |
中国食品药品检定研究院，北京 102629 |
National Institutes for Food and Drug Control, Beijing 102629, China |
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| 中文摘要:目的 通过比较19批不同产地宁夏枸杞果实和3批北方枸杞果实的总糖含量、重均相对分子质量（Mw）及单糖组成，探索枸杞中多糖成分的差异性。方法 采用水提醇沉法获得22批枸杞的粗多糖成分，以葡萄糖为对照品，采用苯酚-硫酸法测定总糖含量；Shodex SB-806 HQ型凝胶柱和Shodex SB-804 HQ型凝胶柱串联，流动相为0.1%氯化钠溶液，柱温为40 ℃，柱流速为0.5 mL·min–1，采用凝胶渗透色谱-示差检测器-多角度激光光散射法（GPC-RI-MALLS）测定Mw及分布系数；选择Zorbax Eclipse XDB C18色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈-磷酸二氢钾溶液，柱温为35 ℃，流速为1.0 mL·min–1，检测波长为250 nm，采用1-苯基-3-甲基-5-吡唑啉酮（PMP）柱前衍生化-高效液相色谱-光电二极管阵列检测器（HPLC-PDA）测定单糖组成，同时建立单糖组成指纹图谱。结果 22批枸杞样品中粗多糖成分的质量分数为3.24%~8.66%，Mw为0.733×106~3.191×106；多糖成分均由9种单糖组成，其中阿拉伯糖含量最高。结果显示，不同产地宁夏枸杞粗多糖结构较为一致，但北方枸杞中多糖成分在结构上与宁夏枸杞存在较明显差异，主要体现在Mw、色谱峰面积比及半乳糖醛酸含量上。结论 宁夏枸杞和北方枸杞的粗多糖成分在结构上有较大差异，且通过建立的单糖组成及Mw测定方法可以对其进行有效区分，可为枸杞质量标准完善及枸杞资源评价提供方法和思路。 |
| 中文关键词:多糖 指纹图谱 单糖组成 重均相对分子质量 宁夏枸杞 北方枸杞 |
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| Determination of Polysaccharides in wolfberry from Different Producing Areas and Species |
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| Abstract:Objective To explore the differences of polysaccharides in Lycium chinense Mill. through the comparison of the total sugar content, weight-average molecular weight (Mw), and monosaccharide composition of 19 batches of fruit of L. barbarum L. samples from different producing areas and 3 batches of L. chinense Mill. var. potaninii (Pojark.) A. M. Lu samples.Methods The crude polysaccharides of 22 batches of samples were extracted by water and precipitated by ethanol. The glucose was used as the reference sample, and phenol-sulfuric acid method was used to determine the total sugar content. The Shodex SB-806 HQ and Shodex SB-804 HQ column were combined with the mobile phase of 0.1% sodium chloride solution, and the column temperature was 40 ℃ with 0.5 mL·min–1 of the flow rate. The gel permeation chromatography coupled to multi-angle laser light scattering and refractive index detectors (GPC-RI-MALLS) were used to determine the Mw and distribution coefficients. The Zorbax Eclipse XDB-C18 (250 mm×4.6 mm, 5 μm) column was chosen with the mobile phase of acetonitrile-potassium dihydrogen phosphate solution, and the column temperature was 35 ℃ with 1.0 mL·min–1 of the flow rate. The detection wavelength was 250 nm, and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization combined high performance liquid chromatography-photo diode array (HPLC-PDA) method was used to determine the monosaccharide composition. At the same time, the fingerprint of monosaccharide composition was established.Results The content of crude polysaccharides in 22 batches of samples was 3.24%-8.66%, and Mw was 0.733×106-3.191×106. The polysaccharides were composed of 9 monosaccharides, among which arabinose content was the highest. Moreover, the results showed that the structure of crude polysaccharide of L. barbarum L. from different producing areas was relatively consistent, but there were obvious differences in the structure of polysaccharides between L. barbarum L. and L. chinense Mill. var. potaninii (Pojark.) A. M. Lu, which mainly reflected in Mw, chromatographic peak area, and the galacturonic acid content.Conclusion There are significant differences in the crude polysaccharides between L. barbarum L. and L. chinense Mill. var. potaninii (Pojark.) A. M. Lu, which can be effectively distinguished by the monosaccharide composition and Mw determination method. This study provides references for the improvement of quality standard and the evaluation of resources of wolfberry. |
| keywords:polysaccharides fingerprinting monosaccharide composition weight-average molecular weight (Mw) Lycium barbarum L. Lycium chinense Mill. var. potaninii (Pojark.) A. M. Lu |
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